摘要
采用厌氧培养箱、气相色谱技术和MPN计数法,研究了甲胺磷和乙草胺在4种浓度(0 1、0 2、0 5和1 0mg·L-1)下对水稻田土壤产甲烷菌种群数量及其活性的影响。加甲胺磷后7d内刺激产甲烷菌种群的生长,数量增加0.2～44.1倍,每管的产甲烷量增加0.8～2.1倍,且刺激作用随药剂浓度的增加而增强;加乙草胺后7d内,产甲烷菌的生长受到抑制,数量下降了50%～99%,每管的产甲烷量显著减少,且抑制作用随药剂浓度的增加而增强。随着加药时间的延长,甲胺磷和乙草胺逐渐降解,产甲烷菌数量又恢复至原有水平。
The technologies of anaerobic culture case,gas chromatography and most probable number method were used to investigate effects of methamidophos and acetochlor(0,0.1,0.2,0.5,and 1.0 mg·L^(-1)) on population and activity of methanogens in paddy soil.The results show that the population of methanogens increased by 0.2-44.1 times,and the production of methane per tube by 0.8-2.1 times within 7 days after methamidophos was added,indicating that the stimulating effect rose with the concentration of the chemical.But the growth of methanogens was inhibited within 7 days after acetochlor was added,with the population decreased by 50%-99%,and the production of methane per tube falling significantly.And the retarding effect increased with the concentration of the pesticide.And the population and activity of methanogens returned to the normal level with the days passing on after the 7th day.
出处
《农村生态环境》
CSSCI
CSCD
北大核心
2004年第3期56-59,共4页
Rural Eco-Environment
