摘要
目的探讨扶正透邪解毒化瘀方对多重耐药铜绿假单胞菌(MDRPA)肺炎老年大鼠不同时相炎症损伤的影响。方法将老年SD大鼠分为空白组、模型组、西药组、中药组、中药提前给药组、中西医结合治疗组,除空白组外使用经口气管插管法进行MDRPA肺炎造模,观察各组大鼠死亡保护率、生命延长率;在感染后不同时间点取大鼠血清及肺组织标本,应用ELISA试剂盒测定血清肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)水平,光镜下观察肺组织支气管、肺泡等结构;在感染后第3日取肺组织标本,电镜下观察各组肺组织超微结构。结果中药提前给药组和中西医结合治疗组可提高死亡保护率和生命延长率。中西医结合治疗组可明显降低MDRPA肺炎老年大鼠血清中促炎因子TNF-α水平,升高抑炎因子IL-10水平,其作用强度明显优于西药组,且可明显减少肺组织炎症细胞浸润和充血水肿,明显减轻肺泡结构的破坏。中药提前给药组可在炎症反应早期升高血清抑炎因子IL-10水平,降低促炎因子TNF-α水平。结论扶正透邪解毒化瘀方对老年MDRPA肺炎大鼠有着较高的死亡保护率和生命延长率,与头孢他啶联合治疗可纠正MDRPA感染后紊乱的炎症状态,减轻炎症损伤。中药提前给药可对MDRPA感染后造成的炎症损伤起到保护与治疗作用,对维持机体免疫稳态具有重要意义。
Objective:To explore the effect of Fuzheng Touxie Jiedu Huayu Decoction on inflammatory damage in elderly rats with multidrug-resistant Pseudomonas aeruginosa(MDRPA)pneumonia at different time points.Methods:Elderly SD rats were divided into blank group(BG),model group,western medicine group,traditional Chinese medicine group,traditional Chinese medicine early administration group,and combination of Chinese and western medicine treatment group.In addition to BG,MDRPA pneumonia models were established by using oral tracheal intubation method,and the death protection rate and life extension rate of rats in each group were observed.Rat serum and lung tissue samples were taken at different time points after infection,and ELISA kits were used to determine the serum TNF-αand IL-10 levels of each group at different times.And each group′s lung tissue,including bronchi and alveoli,were observed under microscopy.On the third day after infection,lung tissue samples were taken and the ultrastructure of lung tissue in each group was observed under electron microscopy.Results:Traditional Chinese medicine early administration group and combination of Chinese and western medicine treatment group had a higher mortality protection rate and life extension rate.The combination of Chinese and western medicine treatment group could significantly reduce the TNF-αlevel in serum and improve the IL-10 level in serum of the elderly rat pneumonia model caused by MDRPA infection,and the effect was better than western medicine group.Meanwhile,it showed a decrease in inflammatory cell infiltration and a significant reduction in lung tissue damage.Traditional Chinese medicine early administration group could increase the anti-inflammatory factor IL-10 in the early inflammatory response and reduce the pro-inflammatory factors TNF-α.Conclusion:Fuzheng Touxie Jiedu Huayu Decoction has a high mortality protection rate and life extension rate for MDRPA pneumonia in the elderly rats.The combination of this formula and ceftazidime can correct the disordered inflammatory state after infection with MDRPA and alleviating lung tissue damage during the most severe inflammatory response.Early administration can alleviate inflammatory reactions in the early stage of infection and play a protective role,which is important in maintaining the body′s immune homeostasis.
作者
刘凤仪
徐红日
林英
马洁
马洪皓
曹鸿云
于淼
张路遥
李鸥
李莹
Liu Fengyi;Xu Hongri;Lin Ying;Ma Jie;Ma Honghao;Cao Hongyun;Yu Miao;Zhang Luyao;Li Ou;Li Ying(Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China)
出处
《中国中医急症》
2024年第1期17-21,共5页
Journal of Emergency in Traditional Chinese Medicine
基金
国家中医药管理局中医药创新团队及人才支持计划项目(ZYYCXTD-D-202208)
北京中医药大学第三附属医院培育项目(BZYSY-2022-PYMS-16)。
关键词
肺炎
多重耐药铜绿假单胞菌
扶正透邪解毒化瘀方
炎症损伤
大鼠
Pneumonia
Multidrug-resistant Pseudomonas aeruginosa
Fuzheng Touxie Jiedu Huayu Decoction
Inflammatory injury
Rats
