摘要
目的评价菊睛丸改善D-半乳糖致亚急性衰老小鼠视网膜损伤效应并探索作用机制。方法采用小鼠皮下注射D-半乳糖构建亚急性衰老模型,分别给予西药阳性药AREDS2、中药阳性药杞菊地黄丸、菊睛丸水提取物低、高剂量组和菊睛丸50%乙醇提取物低、高剂量组。通过光学相干断层成像检查(OCT)检测小鼠视网膜及眼底情况,HE染色法检测视网膜细胞损伤程度,TUNEL染色法检测视网膜细胞凋亡情况,试剂盒检测氧化应激因子表达,Western blot检测小鼠视网膜凋亡和氧化应激通路相关蛋白表达情况。结果菊睛丸提取物显著增加模型小鼠视网膜厚度(P<0.05),改善视网膜损伤并抑制视网膜细胞凋亡(P<0.05)。同时,菊睛丸提取物下调Cleaved Caspase-3与Cleaved Caspase-9凋亡通路蛋白表达(P<0.05),上调Bcl-2/Bax蛋白表达比值(P<0.05)。菊睛丸提取物显著提高抗氧化因子SOD与GSH等表达(P<0.05),减少氧化产物MDA产生(P<0.05),显著上调抗氧化蛋白Nrf2与HO-1的表达(P<0.05)。菊睛丸水提取物和50%乙醇提取物有相似的作用趋势。结论菊睛丸改善D-半乳糖亚急性衰老模型小鼠视网膜损伤,其作用途径与抑制视网膜细胞凋亡并改善氧化应激有关。
OBJECTIVE To evaluate the effect of Jujing Pills on retinal injury in subacute aging mice induced by D-galactose and explore its mechanism.METHODS A subacute aging model was constructed by subcutaneous injection of D-galactose into mice.Western medicine positive drug AREDS2,Chinese medicine positive drug Qiju Dihuang Pills,low and high dose groups of water extract of Jujing Pill,and low and high dose groups of 50%ethanol extract of Jujing Pill were administered,respectively.Optical coherence tomography(OCT)was used to evaluate the condition of mouse retina and fundus,HE staining was adopted to assess retinal cell damage,TUNEL staining was applied to observe retinal cell apoptosis,kits were used to measure the expression of oxidative stress factors,and western blotting was used to detect the expression of retinal apoptosis and oxidative stress pathway related proteins in mice.RESULTS The extracts of Jujing Pills significantly increased the retinal thickness(P<0.05),improved the retinal injury and inhibited the apoptosis of retinal cells in model mice(P<0.05).At the same time,Jujing Pills down-regulated the expression of apoptotic proteins such as Cleaved Caspase-3 and Cleaved Caspase-9(P<0.05)while up-regulated the expression ratio of Bcl-2/Bax(P<0.05).Jujing Pills also significantly increased the expression of antioxidant factors SOD and GSH(P<0.05),decreased the production of oxidative product MDA(P<0.05),and significantly up-regulated the expression of antioxidant proteins Nrf2 and HO-1(P<0.05).The water extracts of Jujing Pills exerted similar retina protection effect with the 50%ethanol extracts.CONCLUSION Jujing Pills can improve the retinal injury of subacute aging model mice,which is related to the inhibition of retinal cell apoptosis and the improvement of oxidative stress.
作者
陆韫青
曹程
郑嘉妮
朱梓强
仝佳祥
齐程成
袁磊
郭盛
宿树兰
康宏杰
段金廒
朱悦
LU Yun-qing;CAO Cheng;ZHENG Jia-ni;ZHU Zi-qiang;TONG Jia-xiang;QI Cheng-cheng;YUAN Lei;GUO Sheng;SU Shu-lan;KANG Hong-jie;DUAN Jin-ao;ZHU Yue(School of Pharmacy,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Jiangsu Province Key Laboratory of High Technology Research,Nanjing University of Chinese Medicine,Nanjing 210023,China;Ningxia Innovation Center of Goji,Yinchuan 750002,China)
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2023年第9期849-859,共11页
Journal of Nanjing University of Traditional Chinese Medicine
基金
国家自然科学基金区域创新发展联合基金重点项目(U21A20408)
宁夏重点研发计划重点项目(2021BEF01003,2021BEF02012,2022BFH02008)
江苏省高等学校基础科学(自然科学)研究重大项目(22KJA360008)
江苏省中药资源产业化过程协同创新中心重点项目(ZDXM-2020-03)
江苏省研究生科研与实践创新计划(KYCX22_2009)。
关键词
菊睛丸
亚急性衰老
视网膜损伤
氧化应激
凋亡
Jujing Pills
subacute aging
retinal injury
oxidative stress
apoptosis
