摘要
试验旨在优化山药叶多酚超声波细胞破碎辅助提取工艺。文章基于单因素试验结果,以超声时间、超声功率、乙醇浓度为影响因素,多酚含量为评价指标,通过响应面法优化提取工艺,采用1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除试验和倍比稀释法测定抗氧化和抑菌活性。试验得到的最佳提取工艺为:超声时间9.0 min、超声功率310 W、乙醇浓度57%、浸提时间50 min、浸提温度60℃、液料比25 mL/g,此时山药叶多酚实际提取量为18.88 mg/g。研究表明,山药叶多酚对DPPH自由基具有较强的清除作用,对大肠杆菌、枯草芽孢杆菌和金黄色葡萄球菌具有较好的抑制效果。
The paper aims to optimize the ultrasound cell disruption auxiliary extraction process of polyphenols from Chinese yam leaves.Based on the results of single-factor experiments,taking ultrasonic time,ultrasonic power and ethanol concentration as the influencing factors,and polyphenol content as the evaluation index,the extraction process was optimized by the response surface method.The antioxidant and bacteriostatic activity were determined by DPPH free radicals removal experiment and double ratio dilution method.The optimal extraction process was as follows:Ultrasonic time of 9.0 min,ultrasonic power of 310 W,ethanol concentration of 57%,extraction time of 50 min,extraction temperature of 60℃,liquid-material ratio of 25 mL/g.Under these condition,the actual extraction amount of yam leaf polyphenols was 18.88 mg/g.The study indicates that polyphenols from Chinese yam leaves have a strong scavenging effect on DPPH free radicals,and have good inhibitory effects on Escherichia coli,Bacillus subtilis andStaphylococcus aureus.
作者
洪永健
胡晶红
刘谦
任鑫
王钰
周成美
祝坤赟
HONG Yong-jian;HU Jing-hong;LIU Qian;REN Xin;WANG Yu;ZHOU Cheng-mei;ZHU kun-yun
出处
《饲料研究》
CAS
北大核心
2023年第17期87-91,共5页
Feed Research
基金
山东省重点研发计划项目(项目编号:2022TZX0035)
日照市重点研发计划项目(项目编号:2021ZDYF010118)
国家现代农业产业技术体系(项目编号:CARS-21)。
关键词
山药叶
多酚
抗氧化活性
抑菌活性
Chinese yam leaves
polyphenol
antioxidant activity
bacteriostatic activity
