摘要
为建立一种增殖系数高、生根效果好的梨矮化砧木中矮1号的组培快繁技术,本试验以中矮1号组培苗为试材,分别对其快繁的外植体种类、消毒方式、继代培养基配方、生根培养基配方和生根培养条件进行研究筛选。结果表明,一年生枝条水培萌发的叶芽和无花芽混合芽更适合作为中矮1号的外植体,最佳消毒方式为:75%酒精30 s→0.1%升汞3 min→无菌水30 s。最佳继代培养基为:MS+30 g/L蔗糖+1.8 mg/L 6-BA+0.1 mg/L NAA+0.2 mg/L GA_(3)+7 g/L琼脂、pH=6.0,平均增殖系数为5.52;6-BA对继代增殖的影响最大,主要影响增殖系数、苗高、叶片数和节间数,NAA主要影响芽长和节间长度,GA_(3)对芽长、苗高和节间长度也有一定影响。两步生根法生根培养效果优:先在配方为1/2MS+15 g/L蔗糖+1.0 mg/L IBA+0.4 mg/L IAA+0.6 mg/L NAA+7 g/L琼脂、pH=6.0的培养基上培养7天(前5天为暗培养),后转入无激素培养基培养,平均生根率达72.22%;活性炭(AC)对生根培养的影响最大,0.5、1.0 g/L AC均会抑制其生根,IBA主要影响生根率,IAA主要影响根数和根长,NAA主要影响生根所需天数。生根培养前期进行暗培养的生根效果要好于强光、弱光、微光培养;刚完成继代培养的幼嫩苗更适合中矮1号的生根培养。本试验建立的中矮1号组培快繁技术,可为优质品种保存和规模化生产提供技术支持。
In order to establish a set of tissue culture and rapid propagation technology for Zhongai 1(S2)pear dwarfing rootstock with high multiplication coefficient and excellent rooting effect,this experiment took the tissue culture seedlings of S2 as materials,and screened the explants,disinfection methods,subcul-ture medium components,rooting medium components and rooting culture conditions for its rapid propagation.The results showed that the mixed buds of leaf buds and non-flower buds germinated in hydroponics of annual branches were more suitable as explants of S2.The best disinfection method was first disinfected with 75%al-cohol for 30 seconds,then disinfected with 0.1%mercuric chloride for 3 minutes,and finally washed with sterile water for 30 seconds.The best subculture medium was MS+30 g/L sucrose+1.8 mg/L 6-BA+0.1 mg/L NAA+0.2 mg/L GA3+7 g/L agar with pH value as 6.0,and the average increment coefficient was 5.52;6-BA had the greatest effect on subculture proliferation,mainly affecting multiplication coefficient,seedling height,leaf number and internode number;NAA mainly affected bud length and internode length;GA3 had a certain effect on bud length,seedling height and internode length.The two-step rooting method had the best rooting culture effect,which was firstly cultured on the medium of 1/2MS+15 g/L sucrose+1.0 mg/L IBA+0.4 mg/L IAA+0.6 mg/L NAA+7 g/L agar with pH value as 6.0 for 7 days(dark culture in the first 5 days),and then cultured on hormone-free medium;the average rooting rate could reach 72.22%.Activated carbon(AC)had the greatest influence on rooting,and both 0.5 and 1.0 g/L AC inhibited its rooting.IBA mainly affected rooting rate,IAA mainly affected root number and root length,and NAA mainly affected the days required for rooting.The rooting effect of dark culture before rooting culture was better compared with cultured in strong light,weak light and low light.The young seedlings from the just completed subculture were more suitable for the rooting culture of S2.The established tissue culture and rapid propagation technology of S2 could provide technical supports for the preservation and large-scale production of high-quality pear varieties.
作者
王艺衡
冯静涵
于春亮
李涛
李金斗
赵健霄
张海霞
张玉星
马辉
许建锋
Wang Yiheng;Feng Jinghan;Yu Chunliang;Li Tao;Li Jindou;Zhao Jianxiao;Zhang Haixia;Zhang Yuxing;Ma Hui;Xu Jianfeng(College of Horticulture,Hebei Agricultual University,Baoding 071051,China;Weixian Bureau of Agriculture and Rural Affairs,Weixian 054700,China;Hebei Pear Technology and Innovation Center,Baoding 071051,China)
出处
《山东农业科学》
北大核心
2023年第6期32-41,共10页
Shandong Agricultural Sciences
基金
国家重点研发计划项目(2019YFD1001404-5)
河北省重点研发计划项目(21326308D-1-4)
河北省海兴梨农业创新驿站项目(2021)
河北省现代农业产业技术体系梨创新团队项目(HBCT2018100202)
广东省重点领域研发计划项目(2020B0202010003)。
关键词
梨砧木
中矮1号
组培快繁
外植体
增殖系数
生根率
Pear rootstock
Zhong’ai 1
Tissue culture and rapid propagation
Explants
Multiplication coefficient
Rooting rate
