摘要
目的探讨血清外泌体长链非编码RNA(LncRNA)00853、LncRNA FAM72D-3在甲胎蛋白(AFP)阴性肝细胞癌(AFP-NHCC)患者中的表达及临床预后意义。方法选取2018年12月至2019年6月该院诊治的AFP-NHCC患者102例为AFP-NHCC组,以同期于该院行健康体检的体检健康者80例为对照组。实时荧光定量PCR检测血清外泌体LncRNA 00853、LncRNA FAM72D-3水平;比较两组血清外泌体LncRNA 00853、LncRNA FAM72D-3水平,分析血清外泌体LncRNA 00853、LncRNA FAM72D-3水平与临床病理特征的关系;采用Kaplan-Meier生存分析血清外泌体LncRNA 00853、LncRNA FAM72D-3对AFP-NHCC患者生存预后的影响;采用单因素及多因素Cox回归分析影响AFP-NHCC患者生存预后的因素。结果AFP-NHCC组血清外泌体LncRNA 00853、LncRNA FAM72D-3相对表达水平分别为3.12±0.86、2.95±0.78,明显高于对照组的1.22±0.36、1.16±0.28(t=17.546、19.552,P<0.05)。血清外泌体LncRNA 00853、LncRNA FAM72D-3与AFP-NHCC患者BCLC分期、病理分化程度及肝内淋巴结转移有关(P<0.05)。LncRNA 00853高表达组患者中位生存时间为29.31个月(95%CI 26.36~32.26)明显低于低表达组患者的32.41个月(95%CI 32.55~34.51),差异有统计学意义(Log-rankχ^(2)=4.844,P=0.028)。LncRNA FAM72D-3高表达组患者中位生存时间为30.43个月(95%CI 27.81~33.04),明显低于低表达组患者的32.61个月(95%CI 30.64~34.76),差异有统计学意义(Log-rankχ^(2)=4.576,P=0.032)。BCLC分期B~C期、病理低分化程度、伴有肝内淋巴结转移、LncRNA 00853高表达及LncRNA FAM72D-3高表达是影响AFP-NHCC患者生存预后的独立危险因素。结论AFP-NHCC患者血清外泌体LncRNA 00853、LncRNA FAM72D-3水平升高,是影响AFP-NHCC生存预后的独立危险因素。
Objective To investigate the expression and clinical prognostic significance of serum exosomal long non-coding RNA(LncRNA)00853 and LncRNA FAM72D-3 in alpha-fetoprotein(AFP)negative hepatocellular carcinoma(AFP-NHCC)patients.Methods A total of 102 patients with AFP-NHCC diagnosed and treated in the hospital from December 2018 to June 2019 were selected as the AFP-NHCC group,and 80 healthy people who underwent physical examination in the hospital during the same period were selected as the control group.Real-time fluorescence quantitative PCR was used to detect the levels of serum exosomal LncRNA 00853 and LncRNA FAM72D-3.The levels of serum exosomal LncRNA 00853 and LncRNA FAM72D-3 were compared between the two groups,and the relationship between serum exosomal LncRNA 00853 and LncRNA FAM72D-3 levels and clinicopathological features was analyzed.Kaplan-Meier survival analysis was used to analyze the effect of serum exosomal LncRNA 00853 and LncRNA FAM72D-3 on the survival prognosis of AFP-NHCC patients.Univariate and multivariate Cox regression analysis were used to analyze the factors affecting the survival prognosis of AFP-NHCC patients.Results The relative expression levels of serum exosomal LncRNA 00853 and LncRNA FAM72D-3 in AFP-NHCC group were 3.12±0.86 and 2.95±0.78,respectively,which were significantly higher than 1.22±0.36 and 1.16±0.28 in control group(t=17.546,19.552,P<0.05).The serum exosomal LncRNA 00853 and LncRNA FAM72D-3 were associated with BCLC stage,pathological differentiation and intrahepatic lymph node metastasis in AFP-NHCC patients(P<0.05).The median survival time of LncRNA 00853 high expression group was 29.31 months(95%CI 26.36-32.26),which was significantly lower than 32.41 months(95%CI 32.55-34.51)of LncRNA 00853 low expression group,and the difference was statistically significant(Log-rankχ^(2)=4.844,P=0.028).The median survival time of LncRNA FAM72D-3 high expression group was 30.43 months(95%CI 27.81-33.04),which was significantly lower than 32.61 months(95%CI 30.64-34.76)of LncRNA FAM72D-3 low expression group,and the difference was statistically significant(Log-rankχ^(2)=4.576,P=0.032).BCLC stage B-C,poor pathological differentiation,intrahepatic lymph node metastasis,high expression of LncRNA 00853 and high expression of LncRNA FAM72D-3 were independent risk factors for survival prognosis of AFP-NHCC patients.Conclusion The elevated serum exosomal LncRNA 00853 and LncRNA FAM72D-3 levels in AFP-NHCC patients are independent risk factors for survival prognosis of AFP-NHCC.
作者
武彩虹
钱成荣
银广悦
侯学伶
韩玉成
田雪梅
WU Caihong;QIAN Chengrong;YIN Guangyue;HOU Xueling;HAN Yucheng;TIAN Xuemei(Department of Clinical Laboratory,Hebei PetroChina Central Hospital,Langfang,Hebei 065000,China)
出处
《国际检验医学杂志》
CAS
2023年第11期1353-1358,共6页
International Journal of Laboratory Medicine
基金
廊坊市科技支撑计划项目(2019013008)。
