摘要
外泌体是一类参与多种生理病理过程的细胞间信息交流载体,其脂质双分子层结构能有效维护所携带的生物信息分子的稳定性。因此,外泌体相对于血清、血浆等更加适合于组学研究,如蛋白质组学、转录组学及代谢组学等。随着质谱技术的不断发展,蛋白质作为诸多生物功能的直接执行者受到越来越多的关注,尤其是外泌体内的蛋白质及其生物功能。外泌体用于蛋白质组学的研究有助于阐明肿瘤的发生和发展机制,进而寻找特异性的肿瘤标志物和可用于精准治疗的靶点。
As carriers of intercellular communication,exosomes are involved in the occurrence and development of a series of physiological and pathological processes.Exosomes have lipid bilayer structure which can effectively protect the stability of bioinformatics molecules inside.Therefore,compared with serum and plasma,exosomes are more conducive to omics research,such as proteomics,transcriptomics and metabolomics.With the development of mass-spectrometic technique,proteins,as the direct executor of many biological functions,have attracted much more attentions,especially the proteins in exosomes and their biological functions.The studies of exosomes in proteomics is helpful to elucidate the mechanism of occurrence and development,so as to find specific tumor markers and targets for precise treatment.
作者
杨柳
张勇
童学东(综述)
邓昆(审校)
YANG Liu;ZHANG Yong;TONG Xuedong;DENG Kun(Department of Clinical Laboratory,the Third Affiliated Hospital of Chongqing Medical University,Chongqing 401120,China)
出处
《国际检验医学杂志》
CAS
2021年第16期2009-2015,共7页
International Journal of Laboratory Medicine
基金
重庆市渝北区科技计划项目[2019(工业)17]
2017重庆市卫生和计划生育委员会医学科研项目(2017ZDXM031)。
关键词
外泌体
蛋白质组学
质谱技术
肿瘤标志物
exosomes
proteomics
mass-spectrometic technique
tumor biomarkers
