摘要
采用HPLC-DAD法研究了不同月份银杏叶色素含量的变化规律,并通过聚类分析和逼近理想值排序(TOPSIS)法评价了不同干燥方式对银杏叶色素含量的影响。研究结果表明:所建立的HPLC-DAD方法简单、准确、灵敏度高、重复性好;4~11月银杏叶色素含量存在明显差异,其中脱植基叶绿素a、脱镁叶绿酸盐a和β-胡萝卜素在4月份达到最高值,分别为(1762±121)、(628±32)和(482±45)μg/g,叶绿素a和b在5月份达到最高值,分别为(8701±571)和(3140±274)μg/g,叶黄素在7月份达到最高值,为(2057±104)μg/g。聚类分析结果表明:12种干燥方式可分为4类,冷冻干燥、40℃减压干燥、40℃常压干燥聚为A类,阴干聚为B类,100℃减压干燥和100℃常压干燥聚为D类,剩下6种干燥方式聚为C类。通过TOPSIS法分析发现:银杏叶干燥方式排名前5的依次为冷冻干燥、40℃减压干燥、40℃常压干燥、阴干和60℃常压干燥。综合考虑,40℃减压干燥是最适合于工业化生产的干燥方法,干燥速度快,且色素得到有效保护。
HPLC-DAD method was used to study the change law of content of Ginkgo biloba leaves pigments in different months,and the effects of various drying methods on the content of G.biloba leaves pigments were evaluated by cluster analysis and technique for order preference by similarity to an ideal solution(TOPSIS).The results showed that the established HPLC-DAD method was simple,accurate,sensitive and reproducible.G.biloba leaves pigments content was significantly different from April to November.The contents of chlorophylide a,pheophorbidel a andβ-carotene reached the highest values in April,which were(1762±121),(628±32)and(482±45)μg/g,respectively.The contents of chlorophyll a and b arrived at the highest values in May,which were(8701±571)and(3140±274)μg/g,respectively.The content of lutein could get to the highest value in July,which was(2057±104)μg/g.Cluster analysis results demonstrated that 12 kinds of drying methods could be divided into four categories.Freeze drying,vacuum drying at 40℃,and atmospheric drying at 40℃were clustered as A category.Shade drying was clustered as B category.vacuum drying at 100℃and atmospheric drying at 100℃were clustered as D category.The left 6 kinds of drying methods were clustered as C category.Through the analysis of TOPSIS method,it was found that the top five drying methods of G.biloba leaves were freeze drying,vacuum drying at 40℃,atmospheric drying at 40℃,shade drying and atmospheric drying at 60℃.Considering comprehensively,vacuum drying at 40℃was the most suitable drying method for industrial production,with fast drying speed and effective protection of pigments.
作者
沈红
张昌伟
陈虹霞
袁花
蒋建新
王成章
SHEN Hong;ZHANG Changwei;CHEN Hongxia;YUAN Hua;JIANG Jianxin;WANG Chengzhang(Institute of Chemical Industry of Forest Products,CAF/Key Lab.of Biomass Energy and Material,Jiangsu Province/Key Lab.of Chemical Engineering of Forest Products,National Forestry and Grassland Administration/National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass,Nanjing 210042,China;Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing Forestry University,Nanjing 210037,China;College of Materials Science and Technology,Beijing Forestry University,Beijing 100083,China)
出处
《林产化学与工业》
CAS
CSCD
北大核心
2023年第2期89-97,共9页
Chemistry and Industry of Forest Products
基金
国家自然科学基金资助项目(32101473)。
