摘要
SQUAMOSA启动子结合蛋白样(SPL)转录因子广泛存在于植物中,主要参与植物生长、发育以及多种生理生化过程。为明确大豆SPL基因家族在染色体上的分布、保守结构域、亚族种类、进化关系等,采用生物信息学方法对大豆SPL家族基因进行分析。本研究从大豆基因组数据库中筛选得到43个SPL基因家族成员,系统发育树分析得到5个亚群。保守结构同源性分析发现,SPL基因家族都含有SBP-box的锌指结构和核定位信号。染色体定位发现除14号染色体没有SPL分布外,其他染色体均有不同数量的SPL基因分布。基因加倍、扩张分析表明,大豆SPL基因家族成员之间不存在串联重复,但是存在36对片段重复,表明部分大豆SPL基因可能是由基因重复产生;大豆与拟南芥SPL基因家族之间有29个基因之间存在共线性关系,表明大豆与拟南芥SPL基因家族之间具有较高同源性。本研究对SPL基因家族的进化分析,将有助于理解大豆SPL基因的结构、功能及进化关系,为深入研究大豆SPL基因的功能提供有利的理论依据。
SQUAMOSA promoter-binding protein-like(SPL)transcription factors widely exist in plants,mainly involved in plant growth,development and a variety of physiological and biochemical processes.In order to clarify the distribution,conserved domains,subfamily types and evolutionary relationships of soybean SPL gene family on chromosomes,bioinformatics methods were used to analyze soybean SPL family genes.In this study,43 members of SPL gene family were screened from soybean genome database,and 5 subgroups were obtained by phylogenetic tree analysis.Conservative structural homology analysis showed that SPL gene family contained SBP-box zinc finger structure and nuclear localization signal.Chromosome mapping showed that except chromosome 14,there were different numbers of SPL genes in other chromosomes.The gene doubling and expansion analysis showed that there was no tandem repeat among the members of soybean SPL gene family,but there were 36 pairs of fragment repeats,indicating that some soybean SPL genes might be generated by gene repeats.There were 29 collinearity genes between soybean and Arabidopsis SPL gene family,indicating that soybean and Arabidopsis SPL gene family had high homology.The results will deepen our understanding of the structure,function and evolution of SPLs and provide a basis for further study to SPLs in soybean.
作者
王文丽
董英山
刘晓冬
王玉民
赵洪锟
袁翠平
齐广勋
王英男
郑梅竹
李玉秋
Wang Wenli;Dong Yingshan;Liu Xiaodong;Wang Yumin;Zhao Hongkun;Yuan Cuiping;Qi Guangxun;Wang Yingnan;Zheng Meizhu;Li Yuqiu(College of Life Sciences,Changchun Normal University,Changchun,130032;Soybean Research Institute,Jilin Academy of Agricultural Science,Changchun,130033)
出处
《分子植物育种》
CAS
北大核心
2023年第3期717-728,共12页
Molecular Plant Breeding
基金
国家自然科学基金青年基金项目(31901566)
吉林省农业科技创新工程项目(KYJF2021JQ010,CXGC-2021DX013)共同资助。
关键词
大豆
SPL
基因家族
生物信息
转录因子
Soybean
SPL
Gene family
Biological information
Transcription factor
