摘要
目的 为从龙胆属植物中挖掘坚龙胆可替代资源提供依据。方法 采用高效液相色谱(HPLC)法对3种龙胆属植物(坚龙胆、头花龙胆、微籽龙胆)根及根茎中4种活性成分(龙胆苦苷、獐牙菜苦苷、獐牙菜苷和苦龙胆酯苷)的含量进行测定;采用超高效液相色谱-电喷雾-四极杆飞行时间高分辨质谱联用(UPLC-ESI-Q-TOF-MS)技术对上述植物根及根茎中化学成分进行鉴定,并进行主成分分析(PCA)。结果 头花龙胆与坚龙胆根及根茎中均含有龙胆苦苷、獐牙菜苦苷、獐牙菜苷和苦龙胆酯苷这4种活性成分,其中头花龙胆和坚龙胆根及根茎中龙胆苦苷含量均是2020年版《中国药典》(一部)限量标准的4倍以上;而微籽龙胆根及根茎中仅检测到獐牙菜苦苷、獐牙菜苷和苦龙胆酯苷,其中的獐牙菜苷和苦龙胆酯苷含量分别是坚龙胆的34.12、8.81倍。通过UPLCESI-Q-TOF-MS技术从3种龙胆属植物根及根茎中共鉴定出33种化合物,主要为环烯醚萜类成分;此外,坚龙胆和头花龙胆中还含有酮类成分,微籽龙胆中还含有黄酮类成分。PCA结果显示,头花龙胆和坚龙胆差异较小,而微籽龙胆与坚龙胆差异较大。结论 同一产地头花龙胆与坚龙胆根及根茎的化学成分差异较小,存在可替代性;而微籽龙胆与坚龙胆根及根茎的化学成分差异较大,不能代替坚龙胆入药。
OBJECTIVE To provide reference for exploring alternative resources of Gentiana rigescens from the plants of Gentiana. METHODS The contents of four components(gentiopicroside, swertiamarin, swertioside and amarogentin) in the roots and rhizomes from 3 plants of Gentiana(G. rigescens, G. cephalantha, G. delavayi) were determined by high-performance liquid chromatography(HPLC). The chemical compositions in the above roots and rhizomes were identified by ultra-performance liquid chromatography electrospray ionization quarter-time of flight mass spectrometry(UPLC-ESI-Q-TOF-MS), and the differences were analyzed by principal component analysis(PCA). RESULTS Four active components such as gentiopicroside, swertiamarin, swertioside and amarogentin were detected in the roots and rhizomes of G. rigescens and G. cephalantha, and the contents of the four components were similar in both. The contents of gentiopicroside in the root and rhizome of G. cephalantha and G.rigescens were more than four times of the limit standard of the Chinese Pharmacopoeia(Part Ⅰ) in 2020;However, only swertiamarin,swertioside and amarogentin were detected in the roots and rhizomes of G. delavayi, and the contents of swertioside and amarogentin were 34.12 and 8.81 times of those of G.rigescens, respectively. In addition, a total of 33 compounds were identified from the roots and rhizomes of 3 plants of Gentiana by UPLC-ESI-Q-TOF-MS, mainly iridoids.Additionally, G. rigescens and G. cephalantha contained xantones, G. delavayi contained flavonoids. PCA showed that there was a small difference between G. rigescens and G. cephalantha;however, there was a big difference between G. delavayi and G. rigescens. CONCLUSIONS The difference between the roots and rhizomes of G. cephalantha and G. rigescens from the same origin is small and there is substitutability;while the difference in the chemical components from roots and rhizomes between G. delavayi and G. rigescens is great and G. delavayi cannot be used as medicine instead of G. rigescens.
作者
李睿
张莹莹
刘卫红
宋艳秋
李龙星
李海峰
LI Rui;ZHANG Yingying;LIU Weihong;SONG Yanqiu;LI Longxing;LI Haifeng(College of Pharmacy,Dali University,Yunnan Dali 671003,China;Yunnan Key Laboratory of Screening and Research on Anti-pathogenic Plant Resources from West Yunnan,Yunnan Dali 671003,China;Library of Dali University,Yunnan Dali 671003,China;College of Public Health,Dali University,Yunnan Dali 671003,China;Analysis and Testing Center,Dali University,Yunnan Dali 671003,China)
出处
《中国药房》
CAS
北大核心
2023年第3期303-308,共6页
China Pharmacy
基金
国家自然科学基金资助项目(No.81960712)
云南省科技计划项目(No.202001BA070001-054)
云南省滇西抗病原植物资源筛选研究重点实验室开放课题立项项目(No.APR202108)。
关键词
坚龙胆
头花龙胆
微籽龙胆
环烯醚萜类成分
化学成分
含量测定
鉴定
Gentiana rigescens
Gentiana cephalantha
Gentiana delavayi
iridoids
chemical components
content determination
identification
