摘要
人类杯状病毒(humancalicivirus,HuCV)是引起儿童和成人非菌性胃肠炎的主要病原之一。为了掌握HuCV在我国的流行情况,1998年7月至2001年6月,从长春市儿童医院2343例5岁以下腹泻患儿中共收集粪便标本1264份,其中1056份来自2135例住院患儿。对轮状病毒检测为阴性的588份标本,经多价酶免疫试验(EIA)和两组引物反转录-聚合酶链反应(RT-PCR)检测HuCV,202份为阳性,其中住院患儿标本178份,HuCV检出率为16 9%。HuCV腹泻以2岁以下儿童为主(占96%),流行高峰季节为11月至次年3月。选择17株HuCV进行分子鉴定,15株属GⅡ 4群,1株属GⅡ 3群,另1株属GⅠ 2群,表明GⅡ 4群HuCV是我国流行的优势株。根据HuCV住院患儿的监测资料初步估计,HuCV腹泻住院率约为0 5‰～2 4‰。讨论了长春地区HuCV的流行趋势和疾病负担。以上结果为我国HuCV腹泻的预防和控制提供了科学依据。
Human calicivirus(HuCV)is one of the important pathogens of viral diarrhea among children and adultsWe conducted hospitalbased HuCV surveillance among children under 5 years of ageBetween July 1998 to June 2001,1,264 fecal specimens were collected from changchun children's hospital of which 1,056 stools were from 2,135 hospitalized children202 HuCVs were identified in 588 rotavirusnegative specimens by using both polyvalent EIA and RTPCR with two sets of primer pairsAmong them,178 HuCVs were from inpatients with a positive rate of 17%(178/1056),24 HuCVs from outpatients with positive rate of 115%(24/208).HuCV detection was the highest among infants aged 12-17 months(30%),followed by 9-11months(22%)and 6-8 months(16%)A consistent epidemic occurred during the winter(November through the next March).Of 17 HuCV strains that were cloned and sequenced,15 belonged to Norwalklike virus(NLV)genotype GⅡ-4,1 was NLV genotype GⅡ-3,and 1 was NLV genotype GⅠ-2,which suggested GⅡ-4 HuCVs were the prevalent strains circulating in ChangchunThe rough estimated rate of HuCVattributed hospitalization was 05/1000-24/1000The present findings provide epidemiological background for prevention and control of HuCV diarrhea in China
出处
《病毒学报》
CAS
CSCD
北大核心
2002年第4期332-336,共5页
Chinese Journal of Virology
基金
美国NIH课题(RO3TWO1192)资助
