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两株诺瓦克样病毒新亚型的鉴定 被引量：2

Identification of two new strains of norovirus in Guangzhou
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摘要 目的对在广州检出的人类杯状病毒(HuCVs)毒株进行分子生物学鉴定,以确定其是否为新亚型。方法选择检出的HuCVs进行克隆、转化和序列分析,使用PHYLIP进行核酸序列同源性比较,经Treeview1.5绘制进化树。结果206份病毒性腹泻标本检出HuCVs18份,检出率为8.74%(18/206),经序列分析证实均为NVs GⅡ毒株。任选其中6株进行PCR产物的克隆和测序,与GenBank中的参考株比较并绘制遗传进化树,其中HuCV/NVGⅡ003/2003/CHN(32282)、HuCV/NVGⅡ004/2003/CHN(32283)与基因文库中病毒的最高同源性分别为83%和87%。结论广州存在其他国家或地区没有报道过的诺瓦克样病毒新亚型毒株。 Objective To identify the molecular biological characteristics of human caliciviruses (HuCVs) and new strains of norovirus (NV) detected in Guangdong Province. Methods HuCVs were detected from the stool specimens by reverse transcription-PCR (RT-PCR), and the PCR products were cloned, transformed, and blasted with Genbank.The nucleotide sequence homology was also evaluated with PHYLIP package, and the phylogenetic tree was generated by Treeview. Results HuCVs were detected from 18 out of 206 stool specimens from children with viral diarrhea (8.74%), which were all confirmed subsequently as NVs. Two strains HuCV/NVGⅡ 003/2003/CHN(32282) and HuCV/NVGⅡ 004/2003/CHN(32283) from the selected 6 NVs strain were identified as new NV strains. Conclusion New NV strains in consistency with the blood-type distribution of Chinese are present in Guangzhou, which have not been reported in other countries.
作者 戴迎春 聂军 刘翼 李志峰 白杨 李建栋 俞守义 江熙
机构地区 第一军医大学流行病学教研室 辛辛那提儿科研究中心
出处 《第一军医大学学报》 CSCD 北大核心 2004年第9期1016-1018,1022,共4页 Journal of First Military Medical University
基金 广东省医学科研基金(A2003420)~~
关键词 人类杯状病毒 诺瓦克样病毒 基因亚型 腹泻 human caliciviruses norovirus sub-genotype diarrhea
分类号 R373 [医药卫生—病原生物学]
  • 相关文献

参考文献12

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二级参考文献10

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共引文献24

同被引文献22

  • 1戴迎春,聂军,刘翼,李志峰,李建栋,俞守义.一起家庭聚集性诺瓦克样病毒感染的调查[J].第四军医大学学报,2004,25(17):1548-1550. 被引量：11
  • 2刘翼,戴迎春,李建栋,詹惠春,陈清,俞守义,聂军.广州地区扎幌样病毒的检出及基因型分析[J].第一军医大学学报,2004,24(10):1147-1149. 被引量：8
  • 3金玉,黄湘,方肇寅,谭家余,王端可,谢华萍,章菁.兰州地区婴幼儿病毒性腹泻的分子流行病学研究[J].中国实用儿科杂志,2006,21(1):15-18. 被引量：57
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引证文献2

二级引证文献14

第一军医大学学报
2004年 第9期

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