摘要
我国普通菜豆的主产区主要分布在干旱、半干旱地区。干旱是影响其生长的主要因素之一。为了筛选抗旱种质资源以及建立抗旱性评价方法,以50份来源不同的普通菜豆品种为材料,采用盆栽法,设正常供水和干旱胁迫2种处理,在干旱胁迫12 d和复水3 d后分别取样测定各项形态和生理指标;采用干旱胁迫和复水后两者的抗旱度量值(D值)、聚类分析和逐步回归方程以及通径分析相结合的方法,对普通菜豆进行苗期的抗旱鉴定及抗旱指标的筛选。结果表明,干旱12 d后超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性,复水3 d后相对叶绿素含量、相对含水量与各自D值显著相关,两处理的丙二醛(MDA)含量与各自D值极显著相关。根据各自D值进行聚类分析,将50份供试材料进行抗旱级别分类,两处理分类结果的一致率达到40%,邻级率为60%,未出现跨级材料;综合两处理的分类结果进一步细分,可分为高抗、中高抗、中抗、中低抗和低抗5个抗旱级别,其中高抗材料10份、中高抗材料25份、中抗材料7份、中低抗材料6份、低抗材料2份。综上所述,普通菜豆苗期干旱胁迫和复水后的D值相互判定的方法可提高鉴定结果的可靠性;干旱胁迫下的SOD活性、POD活性、MDA含量和复水后的相对叶绿素含量、相对含水量、MDA含量可作为普通菜豆苗期抗旱性鉴定的指标,尤其是两者的MDA含量可直接用于普通菜豆资源的抗旱性鉴定。本研究为简化鉴定筛选工作提供了方法和理论依据。
The producing areas of common beans in China was mainly distributed in arid and semi-arid region.Drought is one of the major limiting factors for common beans'production.In this study,two treatments(drought stress and normal water supply)were employed to evaluate the drought resistance of fifty common bean cultivates in pot experiments.The morphological and physiological indexes were determined at 12 days after drought treatment and 3 days after rewatering.A method of combining drought resistance comprehensive evaluation value(D value),clustering analysis,stepwise regression analysis and path analysis were applied to perform the drought resistance identification and drought resistance index selection of common beans at seedling stage.The result showed that the D value were significantly correlated with SOD and POD activity of 12 days after drought treatment and the relative content of chlorophyll,leaf relative water of 3 days after rewatering,and the D value were extremely significantly correlated with the content of MDA of both drought treatment and rewatering.Clustering analysis according to D value can divide 50 materials into different drought resistance grades,the coincidence rate of the two classification results was 40%,the adjacent grade rate was 60%,and there was no cross-grade material.The 50 common beans materials were divided into 5 groups,in which ten varieties were high resistance,twenty-five were medium and high resistance,seven were moderate resistance,six were medium and low resistance and two were low resistance.In conclusion,the combination of D value of drought treatment and rewatering could improve the reliability of drought resistance identification.SOD activity,POD activity,MDA content of drought treatment and the relative content of chlorophyll,MDA content,leaf relative water of 3 days after rewatering could be used as the indexes for indentifing common bean drought resistance identification at seedling stage,MDA content of the two treatments could be directly used in drought resistance identification of common bean resources.The results provide a theoretical reference and method for simplifying the drought resistance indentification of common beam resources.
作者
李洁
张小宁
晋凡生
韩彦龙
李海金
LI Jie;ZHANG Xiaoning;JIN Fansheng;HAN Yanlong;LI Haijin(Shanxi Institute of Organic Dryland Farming,Shanxi Agricultural University/Shanxi Key Laboratory of Organic Dry Farming,Taiyuan,Shanxi 030000)
出处
《核农学报》
CAS
CSCD
北大核心
2022年第8期1516-1529,共14页
Journal of Nuclear Agricultural Sciences
基金
山西省农科院创新项目(YCX2020420)
山西省重点研发计划项目(201803D221012-3)
山西省杂粮产业技术体系建设(杂粮)(2022-03)。
关键词
普通菜豆
苗期
抗旱性
综合评价
common bean
seedling stage
drought resistance
comprehensive evaluation
