摘要
目的 体外细胞实验研究新疆5种阿魏及不同极性部位对人结肠癌HCT 116细胞的增殖抑制、促凋亡作用,体内研究新疆5种阿魏对结肠癌CT-26小鼠的影响。方法 新疆5种阿魏及不同极性部位干预人结肠癌HCT 116细胞,采用MTT比色法检测吸光度OD值,计算增殖抑制率,应用流式细胞术检测细胞凋亡率。建立BALB/c结肠癌CT-26小鼠肿瘤模型,随机分为正常组、模型组、顺铂组和5种阿魏低、高剂量组。连续灌胃给药10 d,第11天处死小鼠,取出肿瘤、脾、肾与胸腺,计算各组小鼠的脏器指数与抑瘤率。结果 对于人结肠癌HCT 116细胞,多伞阿魏、准噶尔阿魏、新疆阿魏的乙醇提取物抑制作用较好,IC分别为26.06、28.31、42.27μg/mL,托里阿魏抑制作用较弱,IC为131.61μg/mL,圆锥茎阿魏作用最弱,IC为261.77μg/mL;准噶尔阿魏石油醚部位、二氯甲烷部位、乙酸乙酯部位对人结肠癌HCT 116细胞的抑制作用均很明显,水饱和正丁醇部位抑制作用较弱,而水提部位几乎没有作用。新疆5种阿魏中,准噶尔阿魏对于人结肠癌HCT 116细胞的促凋亡作用较好。对于结肠癌CT-26小鼠,与正常组相比,新疆5种阿魏给药组小鼠的脾重、肾重、胸腺重及其脏器指数均有降低趋势,与阳性药物顺铂组作用趋势一致;从抑瘤效果来看,准噶尔阿魏高低剂量组抑瘤率分别为61.73%、67.40%。结论 准噶尔阿魏抗结肠癌作用较好,值得更进一步的深入研究。
Objective In vitro cell experiments were conducted to study the proliferation-inhibition and apoptosis effects of five species of Ferula from Xinjiang and several polar regions on human colon cancer HCT 116 cells, and an in vivo study examined the effects of five species of Ferula from Xinjiang on colon cancer CT-26 mice. Methods Human colon cancer HCT 116 cells were treated with five species of Ferula from Xinjiang and several polar regions. The optical density absorbance was measured by MTT method, the proliferation-inhibiting rate was calculated, and the cell apoptosis rate was measured by flow cytometry. A BALB/c colon cancer CT-26 mice tumor model was established and randomly divided into a normal, model, cisplatin and Ferula species high-dose and low-dose groups. On the 11day, the mice were sacrificed, the tumor, spleen, kidneys, and thymus were removed, and the organ index and tumor inhibition rate of each group were calculated. Results For HCT 116 cells, the inhibitory ICvalues of Ferula ferulaeoides, F. songorica, and F. sinkiangensis were 26.06, 28.31 and 42.27 μg/mL, respectively, while the inhibitory effect of F. krylovii was weak, with an ICof 131.61 μg/mL. F. conocaula had the weakest inhibitory effect, with an ICof 261.77 μg/mL. Petroleum ether, methylene chloride, and ethyl acetate extracts of F. songorica had obvious inhibitory effect on HCT 116 cells, but the water-saturated n-butanol extract had only a weak inhibitory effect, and the water extract had almost no inhibitory effect. Among the five species of Ferula found in Xinjiang, F. songorica had the best apoptotic effect on HCT 116 cells. Compared with the normal group, colon cancer CT-26 mice treated with Ferula tended to have decreased spleen, kidney, and thymus weights and organ index, which is consistent with the positive-control cisplatin drug group. In terms of tumor inhibition effect, the tumor inhibition rates of F. songorica high-and low-dose groups were 61.73% and 67.40%, respectively. Conclusions The anti-tumor effect of F. songorica is promising and worthy of further study.
作者
张柏生
郭萍萍
叶晨雨
张海英
ZHANG Bosheng;GUO Pingping;YE Chenyu;ZHANG Haiying(the Affiliated Traditional Chinese Medicine Hospital,Xingjiang Medcial University,Urumqi 830000,China;the Fourth College of Clinical Medicine,Xinjiang Medical University,Urumqi 830000;Xinjiang Key Laboratory of Processing and Research of Traditional Chinese Medicine,Urumqi 830000)
出处
《中国比较医学杂志》
CAS
北大核心
2022年第11期26-33,共8页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金资助项目(82060734)。
关键词
结肠癌
增殖抑制
凋亡
准噶尔阿魏
colon cancer
inhibiting proliferation
apoptosis
Ferula songorica
