摘要
为明确我国部分地区绵羊与山羊戊型肝炎病毒(HEV)的感染状况,采用双抗原夹心酶联免疫法(DS-ELISA)检测采自我国20个省、自治区、直辖市的3 324份羊血清中的HEV抗体,比较分析不同地区羊群HEV抗体阳性率的差异;同时对血清阳性样本进行巢式RT-PCR检测,并对克隆所获得的PCR产物连接T载体后进行测序分析。结果显示,我国20个省、自治区、直辖市羊血清中HEV抗体总阳性率为13.27%(441/3 324),其中新疆、四川阳性率较高,分别为23.68%、22.1%;内蒙古、甘肃阳性率较低,分别为2.49%、2.16%。对血清学阳性样品进行巢式RT-PCR检测,检出8个阳性样本,经ORF2部分基因序列分析,构建进化树,发现其中6个样本中的病毒属于Gt 4H,其余2个样本中的病毒属于Gt 4D。说明我国羊群中存在广泛的HEV感染,且主要流行基因型为基因4型。
To definite the infection status of hepatitis E Virus(HEV) in sheep and goats in some regions in China,the double antigen sandwich ELISA(DS-ELISA) was used to detect HEV antibodies in 3 324 sera(including sheep and goats) from 20 regions in China,and the differences in the positive rate of HEV antibodies in different regions were compared and analyzed;at the same time,nested RT-PCR was performed on seropositive samples to detect HEV gene,and sequencing and analysis of ORF2 gene obtained from positive samples.The results showed that the total positive rate of HEV antibodies in goat serum from 20 regions in China was 13.27%(441/3 324),of which Xinjiang and Sichuan had higher positive rate of 23.68% and 22.10%,respectively;Inner Mongolia and Gansu had lower positive rate of 2.49% and 2.16%,respectively.Nested RT-PCR showed that 8 gene positive samples were obtained.After ORF2 gene sequencing and phylogenetic tree analysis,it was found that 6 of them belonged to Gt4 H,and the remaining 2 genes belonged to Gt4 D.There was widespread HEV infections in flocks of sheep and goats in China,and the main prevalent genotype is genotype 4.
作者
胡晗
李胜
丛日华
HU Han;LI Sheng;CONG Rihua(Shaanxi Animal Disease Prevention and Control Center,Xi’an Shaanxi 710016,China;Shaanxi General Station of Animal Husbandry Technology Extension,Xi’an Shaanxi 710016,China 3.Northwest A&F University,Yangling Shaanxi 712100,China)
出处
《中兽医医药杂志》
CAS
2022年第4期62-65,共4页
Journal of Traditional Chinese Veterinary Medicine
基金
陕西省科技厅国际科技合作计划项目(2021KWZ-22)。
