摘要
采用响应面法对诱导富集三萜类化合物灵芝酸A的液态培养基配方进行优化,得到最优配方为:葡萄糖35 g,组氨酸0.7 g,复合VB 40 mg(VB_(1)6 mg,VB_(2)3 mg,VB_(6)0.4 mg,烟酰胺20 mg,泛酸钙2 mg),牛磺酸0.1 g,蒸馏水1000 mL。用5%NaOH以及5%HCl调节pH至6.4。在26℃、160 r·min^(-1)的条件下,此培养基上灵芝菌丝生物量为11.45 g·L^(-1),灵芝酸A的产量为137.98 mg·L^(-1)。
The formula of liquid culture medium,which induced the enrichment of Ganoderma triterpenoid ganoderic acid A,was optimized using the response surface method.The optimal formula was as followed:glucose 35 g,histidine 0.7 g,complex B vitamins 40 mg(VB_(1)6 mg,VB_(2)3 mg,VB_(6)0.4 mg,niacinamide 20 mg,calcium pantothenate 2 mg),taurine 0.1 g,and distilled water 1000 mL.Adjusting pH to 6.4 with 5%NaOH and 5%HCl.At 26℃and 160 r·min^(-1),the mycelium biomass of Ganoderma lucidum on the medium was 11.45 g·L^(-1),and the yield of ganoderic acid A was 137.98 mg·L^(-1).
作者
丁舒
宋兆伟
陈颖
张志军
张峻
陈晓明
DING Shu;SONG Zhao-wei;CHEN Ying;ZHANG Zhi-jun;ZHANG Jun;CHEN Xiao-ming(Institute of Food Storage and Process Scientific Technology,Tianjin Academy of Agricultural Sciences,Tianjin 300384,China)
出处
《保鲜与加工》
CAS
2022年第5期43-48,共6页
Storage and Process
基金
天津市农业科学院青年科研人员创新研究重点项目(2020003)
天津市林业果树研究所所长基金项目(SZJJ2016002)。
关键词
灵芝
液态发酵
培养基优化
响应面法
灵芝酸A
Ganoderma lucidum
liquid fermentation
optimization of culture medium
response surface method
ganoderic acid A
