摘要
目的探讨冻干血小板裂解液(PL)对大鼠关节软骨损伤模型的修复作用。方法SD大鼠25只,分别于实验d1、d3、d5对大鼠行右膝关节腔注射Ⅱ型胶原酶[200μL/(只·次)],末次注射胶原酶后d14观察造模情况,将在≤14 d造模成功大鼠随机均分为冻干PL注射组[A组,注射1 mL/(只·次)],PL组(B组,注射1 mL/(只·次)]及生理盐水对照组[C组,注射1 mL/(只·次)];3组大鼠于治疗的d0、d7、d14、d21分别注射相应药物,观察比较3组大鼠膝关节直径的变化并于治疗的d14、d28从各组随机处死1只动物,取关节腔2个/只,作苏木精-伊红染色(HE)和免疫组织化学染色观察。结果Ⅱ型胶原酶注射造模14 d时大鼠造模成功的比例为84%(21/25),造模前后其膝关节直径(mm)12.84±1.14 vs 14.11±1.17(P<0.01);A、B、C 3个组大鼠在注射不同治疗液的d14、d21和d28时膝关节直径(mm)为13.33±1.16 vs 13.37±1.08 vs 14.21±1.08、13.10±1.09 vs 13.01±1.04 vs 14.09±1.09和12.38±1.08 vs 12.51±1.03 vs 14.01±1.07(均为P<0.05)。组织学观察显示:软骨细胞生成、细胞排列,Ⅱ型胶原蛋白的阳性表达等,A、B 2个大鼠组无明显变化,但均优于C组。结论冻干PL对大鼠关节软骨损伤模型的治疗修复作用具有与PL相近的疗效。
Objective To discuss the repair effect of lyophilized platelet lysate(PL)products on articular cartilage injury model of rats.Methods A total of 25 SD rats were injected with typeⅡcollagenase at the right knuckle articular cavity respectively on day 1,3 and 5 of experiment,and the modeling conditions were observed 14 days after the last injection of collagenase.The SD rats with successful modeling were randomly divided into three groups,and were injected with lyophilized PL[Group A,1 mL/(mouse·time)],PL[Group B,1 mL/(mouse·time)],and normal saline[Group C,1 mL/(mouse·time)].The above three substances were injected with corresponding drugs on day 0,7,14 and 21 of experiment based on the grouping conditions,and the changes of knee joint diameters of the rats from the three groups were observed and compared.On day 14 and 28,one rat in each group was randomly killed and two knuckle articular cavities of each were taken for tissue sampling,using hematoxylin-eosin staining(HE)and immunohistochemistry.Results After 14 days of modeling by injection of typeⅡcollagenase,the proportion of successful modeling in rats was 84%(21/25),with the knee joint diameter(mm)before and after modeling at 12.84±1.14 vs 14.11±1.17(P<0.01).On day 14,21 and 28,groups A and B were superior to group C in the knee joint diameter and activity improvement(P<0.05),with 13.33±1.16 vs 13.37±1.08 vs 14.21±1.08,13.10±1.09 vs 13.01±1.04 vs 14.09±1.09 and 12.38±1.08 vs 12.51±1.03 vs 14.01±1.07,respectively.Histological observation showed that group A and B were superior to group C in the production and arrangement of chondrocytes and the positive expression of typeⅡcollagen,and there was no significant difference between group A and group B.Conclusion The lyophilized PL has similar therapeutic effect to PL in the treatment and repair of articular cartilage injury,and is worthy of clinical application.
作者
刘广亚
许育兵
张喻
朱展鸿
周谋
张卫
单桂秋
LIU Guangya;XU Yubing;ZHANG Yu;ZHU Zhanhong;ZHOU Mou;ZHANG Wei;SHAN Guiqiu(epartment of Blood Transfusion,General Hospital of Southern Theater Command,Guangzhou 510010,China)
出处
《中国输血杂志》
CAS
2022年第4期392-395,共4页
Chinese Journal of Blood Transfusion
基金
广东省医学科学技术研究基金(A2020217)
广州市科技计划项目(202102080531)。
关键词
血小板裂解液
冻干
关节软骨损伤
动物模型
SD大鼠
软骨损伤修复
platelet lysates
lyophilized
articular cartilage injury
animal model
SD rats
repair of cartilage injury
