摘要
目的:探讨紫草素对慢性皮肤溃疡大鼠间隙连接蛋白家族(Cx)的蛋白表达及基因表达水平的影响。方法:SPF级健康SD大鼠42只,选择30只大鼠进行慢性皮肤溃疡造模,筛选造模成功24只大鼠模型进入后续实验,随机分为模型组、紫草素组,每组12只,剩余12只未造模大鼠作为假手术组。造模1周后进行药物干预,模型组给予重组牛碱性成纤维细胞生长因子纱条,紫草素组给予4 mg/mL紫草混悬液纱条,假手术组给予等量生理盐水。每日换药1次,连续给药12 d。在干预7、12 d后观察创面愈合情况;干预结束后取溃疡肉芽组织标本,采用HE染色观察病理组织学变化;WesternBlot法检测Cx26、Cx30、Cx43蛋白表达变化;RT-PCR法检测Cx26、Cx30、Cx43 mRNA相对表达情况。结果:与本组干预7 d后比较,模型组、紫草素组大鼠干预12 d后的创面愈合率均升高(P<0.05);与模型组同时间点比较,紫草素组干预7、12 d后的创面愈合率差异无统计学意义(P>0.05)。HE染色显示,紫草素组干预7、12 d后创面愈合情况均优于模型组。模型组、紫草素组Cx26、Cx30、Cx43蛋白和mRNA表达均低于假手术组(P<0.05);紫草素组Cx26、Cx30、Cx43蛋白和mRNA表达均高于模型组(P<0.05)。结论:紫草素治疗慢性皮肤溃疡大鼠效果明显,可加速创面愈合,其作用机制可能与提高Cx26、Cx32、Cx43水平有关。
Objective:To investigate the effect of shikonin on the protein and gene expressions of connexin family(Cx)in rats with chronic skin ulcer.Methods:30 rats were chosen from SPF healthy SD rats to establish the model of chronic skin ulcer.24 successful modeling rats were randomly divided into the model group and the shikonin group,with 12 rats in each group.The remaining 12 rats were assigned into the sham operation group.Drug intervention was carried out one week after modeling.The model group was given recombinant bovine basic fibroblast growth factor yarn,the shikonin group was given shikonin suspension yarn(4 mg/ml),and the sham operation group was given the same amount of normal saline.The dressing was changed once a day for 12 days.The wound healing was observed 7 and 12 days after the intervention.The ulcer granulation tissue samples were collected after the intervention.The histopathological changes were observed by HE staining,the protein expressions of Cx26,Cx30 and Cx43 were detected by Western blot,and the relative mRNA expressions of Cx26,Cx30 and Cx43 were detected by RT-PCR.Results:The wound healing rate was significantly higher on 12th day than that on 7th day in the model group and in the shikonin group(P<0.05).There was no statistical difference in the wound healing rate between the model group and the shikonin group on 7th and 12th day(P>0.05).HE staining showed that the wound healing rate of the shikonin group was better than that of the model group on 7th day and 12th day.The protein and mRNA expressions of Cx26,Cx30 and Cx43 were lower in the model group and in the shikonin group than those in the sham operation group(P<0.05).The protein and mRNA expressions of Cx26,Cx30 and Cx43 were significantly higher in the shikonin group than those in the model group(P<0.05).Conclusion:Shikonin is effective in the treatment of chronic skin ulcer in rats,and its mechanism may be related to increasing the levels of Cx26,Cx32 and Cx43.
作者
杜伟鹏
张现峰
王晓歌
马立人
DU Weipeng;ZHANG Xianfeng;WANG Xiaoge;MA Liren(Pingdingshan Hospital of Traditional Chinese Medicine,Pingdingshan 467000,China)
出处
《中医药信息》
2022年第3期50-54,共5页
Information on Traditional Chinese Medicine
基金
河南省中医药科学研究专项课题(2017ZY3034)。
