摘要
试验旨在研究没食子酸(GA)和三甲胺N-氧化物(TMAO)对体外瘤胃发酵和三甲胺(TMA)代谢的影响。通过瘤胃体外模拟试验分析瘤胃底物消失率、总产气量、发酵参数和TMA代谢情况,设置对照组、15 mg GA/g DM组、5 mg TMAO/g DM组、5 mg TMAO+15 mg GA/g DM组,每组4个重复,培养时间24 h。结果表明:与对照组相比,添加TMAO有提高24 h瘤胃DM消失率(DMD)的趋势(P=0.08),提高了粗蛋白消失率(CPD)(P<0.01)、总产气量(P=0.02)和累积三甲胺氮(TMA-N)浓度(P<0.01);与对照组相比,添加GA降低了CPD(P<0.01),对累积TMA-N浓度没有影响(P>0.05);与TMAO组相比,同时添加TMAO和GA降低了CPD和累积TMA-N浓度(P<0.01)。由此可见,TMAO促进了体外瘤胃发酵,并提高了TMA浓度,GA对底物中TMA前体物质代谢产生TMA的影响很小,但降低了TMAO瘤胃代谢产生的TMA。
The objective of this study was to investigate the effects of gallic acid(GA)and trimethylamine N-oxide(TMAO)on rumen fermentation and trimethylamine(TMA)metabolism Metabolism.The substrate disappearance rate,total gas production,fermentation parameters and TMA metabolism were analyzed by an Metabolism rumen-simulated experiment.Four treatment groups were set up:control,15 mg GA/g DM,5 mg TMAO/g DM,5 mg TMAO+15 mg GA/g DM with 4 replicates within each group.The incubation time was 24 h.The results showed that added TMAO tended to increase 24 h ruminal disappearance rate of DM(DMD)(P=0.08),and increased disappearance rate of crude protein(CPD)(P<0.01),total gas production(P=0.02)and cumulative concentration of TMA-N(P<0.01),GA addition decreased CPD(P<0.01)and had no effect on cumulative concentration of TMA-N(P>0.05),compared with the control group..Compared with the TMAO group,adding both TMAO and GA decreased CPD and cumulative concentration of TMA-N(P<0.05).Therefore,TMAO promoted Metabolism rumen fermentation and simultaneously increased TMA concentration.GA had little effect on TMA production derived from substrate,but decreased TMA produced by rumen metabolism of TMAO.
作者
魏晨
成海建
姜富贵
靳青
宋恩亮
WEI Chen;CHENG Haijian;JIANG Fugui;JIN Qing;SONG Enliang(Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Shandong Jinan 250100,China;Shandong Provincial Key Laboratory of Animal Disease Control and Breeding,Shandong Jinan 250100,China)
出处
《中国畜牧杂志》
CAS
北大核心
2022年第1期214-218,共5页
Chinese Journal of Animal Science
基金
山东省自然科学基金(ZR2021MC047)
现代农业(肉牛牦牛)产业技术体系专项(CARS-37)
山东省农业科学院农业科技创新工程(CXGC2018E10)。
