摘要
背景:制备可降解材料骨组织工程支架,应用于修复骨肉瘤造成的骨缺损是当前骨组织工程研究热点。目的:确定壳聚糖/聚乳酸/羟基磷灰石/聚乙烯醇复合材料的配比,优化骨支架力学性能。方法:基于挤出式3D打印技术制备壳聚糖/聚乳酸/羟基磷灰石/聚乙烯醇骨支架,以最大抗压强度为评价指标,设计正交实验,以乙酸浓度(1.5%,2%,2.5%,3%)、壳聚糖溶液浓度(2%,3%,4%,5%)、羟基磷灰石∶聚乳酸质量比(1∶5,1∶6,1∶7,1∶8)、羟基磷灰石/聚乳酸∶壳聚糖溶液∶聚乙烯醇凝胶质量体积比(1 g∶1 g∶0 mL,2 g∶1 g∶1 mL,2 g∶1 g∶1.2 mL,2 g∶1 g∶1.4 mL)四因素为自变量,得到最佳材料配比。表征最佳配比复合材料的力学性能、物相组成与结合方式、孔隙率与降解性能。结果与结论:①正交实验结果显示,乙酸浓度为3%、壳聚糖溶液浓度为5%、羟基磷灰石∶聚乳酸质量比=1∶6、羟基磷灰石/聚乳酸∶壳聚糖溶液∶聚乙烯醇凝胶=2 g∶1 g∶1.4 mL时,复合材料具有良好的力学性能;②最佳配比复合材料支架具有良好的连通性,孔隙率为(52.0±1.5)%,最大抗压强度达6 MPa,X-射线衍射扫描及傅里叶红外光谱测试结果显示,复合材料的主要结合方式为物理共混;复合材料支架具有一定的降解性能,但在测试时间内失重率较低,降解性能有待进一步提高;③结果表明,壳聚糖/聚乳酸/羟基磷灰石/聚乙烯醇复合材料支架具有较高的孔隙率和良好的力学性能。
BACKGROUND:Bone tissue engineering scaffolds are prepared and applied to repair bone defects caused by osteosarcoma disease,which is the current hotspot of bone tissue engineering research.OBJECTIVE:To determine the ratio of chitosan/polylactic acid/hydroxyapatite/polyvinyl alcohol composite material,and optimize the mechanical properties of bone scaffolds.METHODS:The chitosan/polylactic acid/hydroxyapatite/polyvinyl alcohol bone scaffold was prepared using extrusion-based 3D printing technology.The maximum compressive strength was used as the evaluation index to design orthogonal experiments.The acetic acid concentration(1.5%,2%,2.5%,3%),chitosan solution concentration(2%,3%,4%,5%),hydroxyapatite:polylactic acid mass ratio(1:5,1:6,1:7,1:8),and hydroxyapatite/polylactic acid:chitosan solution:polyvinyl alcohol gel mass volume ratio(1 g:1 g:0 mL,2 g:1 g:1 mL,2 g:1 g:1.2 mL,2 g:1 g:1.4 mL)were independent variables to get the best material ratio.The mechanical properties,phase composition and bonding mode,porosity and degradation properties of the optimal ratio composite material were characterized.RESULTS AND CONCLUSION:(1)Orthogonal experiment results exhibited that the concentration of acetic acid was 3%;the concentration of chitosan solution was 5%;the mass ratio of hydroxyapatite:polylactic acid was 1:6;the hydroxyapatite/polylactic acid:chitosan solution:polyethylene alcohol gel was 2 g:1 g:1.4 mL.The composite material had good mechanical properties.(2)The composite material scaffold with the best ratio had good connectivity;the porosity was(52.0±1.5)%;and the maximum compressive strength reached 6 MPa.X-ray diffraction scanning and Fourier infrared spectroscopy test results demonstrated that the main bonding method of composite materials was physical blending.Composite scaffolds had certain degradation properties,but the weight loss rate was low during the test time,and degradation property needs to be further improved.(3)These findings indicate that the chitosan/polylactic acid/hydroxyapatite/polyvinyl alcohol composite scaffold has high porosity and good mechanical properties.
作者
李耀明
姜宏
石永芳
Li Yaoming;Jiang Hong;Shi Yongfang(School of Mechanical Engineering,Xinjiang University,Urumqi 830017,Xinjiang Uygur Autonomous Region,China;School of Medical Engineering and Technology,Xinjiang Medical University,Urumqi 830017,Xinjiang Uygur Autonomous Region,China)
出处
《中国组织工程研究》
CAS
北大核心
2022年第18期2888-2893,共6页
Chinese Journal of Tissue Engineering Research
基金
新疆维吾尔自治区自然科学基金项目(2018D01C148),项目负责人:石永芳。
关键词
正交实验
方差分析
挤出式3D打印
骨组织工程支架
力学性能
降解性能
失重率
孔隙率
orthogonal experiment
analysis of variance
extrusion-based 3D printing
bone tissue engineering scaffold
mechanical properties
degradation property
weight loss rate
porosity
