摘要
目的研究上调基因(URG)11及其蛋白在甲状腺乳头状癌组织中的表达及临床意义。方法采用实时定量PCR分别检测甲状腺乳头状癌组织和结节性甲状腺肿组织的URG11基因的表达情况;采用免疫组织化学法分别检测甲状腺乳头状癌组织和结节性甲状腺肿组织中的URG11蛋白的表达情况;分析URG11蛋白在甲状腺乳头状癌组织中的表达和患者的性别、年龄、淋巴结有无转移、临床分期的关系。结果实时定量PCR结果显示URG11基因在甲状腺乳头状癌组织高表达,相对于结节性甲状腺肿组织,相对表达量为2.36±0.03,差异有统计学意义(t=2.21,P<0.05);免疫组织化学法结果显示URG11蛋白在甲状腺乳头状癌组织中的阳性率为71.43%,在结节性甲状腺肿组织中的阳性率为24.00%,差异有统计学意义(χ^(2)=11.85,P<0.05);甲状腺乳头状癌组织中URG11蛋白的表达与患者的年龄、性别、淋巴结转移均不相关(χ^(2)分别=0.44、1.57、0.93,P均>0.05),与患者的临床分期相关(χ^(2)=4.46,P<0.05)。结论甲状腺乳头状癌中URG11在基因和蛋白水平均呈高表达,提示URG11在甲状腺乳头状癌中可能起癌基因作用,为甲状腺乳头状癌的早期诊断提供分子生物学依据。
Objective To investigate the expression and clinical significance of URG11 gene and its protein in papillary thyroid carcinoma. Methods The expression of URG11 gene in papillary thyroid carcinoma tissues and nodular goiter tissues were detected by RT-PCR,and the expression of URG11 protein in papillary thyroid carcinoma tissues and nodular goiter tissues were detected by immunohistochemistry.The relationship between the expression of URG11 protein in papillary thyroid carcinoma tissues and patients’ gender,age,lymph node metastasis and clinical stage were analyzed.Results RT-PCR results showed that URG11 gene was expressed highly in papillary thyroid carcinoma tissues,compared with nodular goiter tissues,the relative expression level was 2.36±0.03,the difference was statistically significant(t=2.21,P<0.05).Immunohistochemical results showed that the positive rate of URG11 protein in papillary thyroid carcinoma tissues was 71.43%,and the positive rate in nodular goiter tissues was 24.00%,the difference was statistically significant(χ^(2)=11.85,P<0.05).The expression of URG11 protein in papillary thyroid carcinoma tissues was not correlated with age,gender and lymph node metastasis of the patients(χ^(2)=0.44,1.57,0.93,P>0.05),but correlated with the clinical stage of patients(χ^(2)=4.46,P<0.05). Conclusion URG11 is highly expressed in papillary thyroid carcinoma at gene and protein levels.It suggested that URG11 may play an oncogene role in papillary thyroid carcinoma,which provided molecular biological basis for early diagnosis of papillary thyroid carcinoma.
作者
权明明
陈珍珍
QUAN Mingming;CHEN Zhenzhen(Department of Surgical Oncology,Taizhou Center hospital,Taizhou 318000,China)
出处
《全科医学临床与教育》
2022年第1期15-17,21,共4页
Clinical Education of General Practice
基金
台州市科技局项目(20ywa26)。
