摘要
背景:胶原/壳聚糖支架需交联才能达到相应力学性能,有研究表示调节交联剂浓度可以在一定范围内调控支架的理化性能。目的:探究京尼平浓度对胶原/壳聚糖支架理化性能的影响,制备理化性能可调节的组织工程支架。方法:将胶原和壳聚糖粉末分别溶于弱酸后混合均匀,作为打印墨水,利用生物3D打印机低温打印胶原支架与胶原/壳聚糖支架,经冻干、中和处理后分别以1,3,5 mmol/L的京尼平进行交联。检测各组支架的表观结构稳定性、抗拉能力、溶胀性能、降解性能与生物相容性。结果与结论:①将支架在PBS中浸泡3 d后,对比未交联的冻干支架,交联后胶原支架表面维持规则的孔结构,但是支架出现明显变形;交联后胶原/壳聚糖支架表面结构规则,仅1 mmol/L京尼平交联的胶原/壳聚糖支架存在轻微变形。②随着京尼平浓度的增加,各组支架的力学性能增加,并且对应交联浓度下的胶原/壳聚糖支架力学性能好于胶原支架。③随着京尼平浓度的增加,胶原支架的溶胀率下降,胶原/壳聚糖支架的溶胀率无明显变化。④浸泡于胶原酶溶液中后,不同浓度京尼平交联的胶原支架在1 h内被完全降解,胶原/壳聚糖支架的降解速率随京尼平浓度的增加而降低,均呈现先快速后平缓的趋势。⑤将骨髓间充质干细胞接种于各组交联支架3 d后,1,3 mmol/L京尼平交联的胶原/壳聚糖支架(或胶原支架)上的细胞数量明显多于5 mmol/L京尼平交联的胶原/壳聚糖支架(P<0.05)。⑥结果表明,京尼平可在一定范围调节胶原/壳聚糖支架理化性能,其中3 mmol/L京尼平交联的胶原/壳聚糖支架具有较好的力学性能、抗酶解能力与生物相容性。
BACKGROUND:Collagen/chitosan scaffolds need to be cross-linked to achieve corresponding mechanical properties.Studies have shown that the regulation of crosslinking agent concentration can control the physical and chemical properties of collagen/chitosan scaffolds.OBJECTIVE:To explore the effect of genipin concentration on physical and chemical properties of collagen/chitosan scaffolds,and manufacture tissue engineering scaffolds with adjustable physical and chemical properties.METHODS:Collagen and chitosan were separately dissolved in weak acid and then mixed as printing ink.The collagen/chitosa scaffolds were printed using biological 3D printer at low-temperature,then lyophilized and neutralized,and crosslinked with 1,3 and 5 mmol/L genipin.The apparent structural stability,tensile strength,swelling performance,degradation performance and biocompatibility of scaffolds of each group were detected.RESULTS AND CONCLUSION:(1)After the scaffold was soaked in PBS for 3 days,compared with the uncrosslinked freeze-dried scaffold,the surface of the collagen scaffold maintained regular pore structure after crosslinking,but the scaffold showed obvious deformation.The surface structure of the collagen/chitosan scaffold was regular after crosslinking.The collagen/chitosan scaffold cross-linked with only 1 mmol/L genipin had slight deformation.(2)With the increased concentration of genipin,the mechanical properties of scaffolds of each group increased,and the mechanical properties of collagen/chitosan scaffolds at the corresponding crosslinking concentration were better than those of collagen scaffolds.(3)With the increased concentration of genipin,the swelling rate of the collagen scaffold decreased,and the swelling rate of the collagen/chitosan scaffold did not remarkably change.(4)After soaking in collagenase solution,the collagen scaffolds crosslinked with different concentrations of genipin were totally degraded within 1 hour.The degradation rate of collagen/chitosan scaffold decreased with the increased concentration of genipin,showing a trend of rapid first and then smooth.(5)After inoculating bone marrow mesenchymal stem cells on the crosslinked scaffolds for 3 days,the number of cells on the 1 and 3 mmol/L genipin crosslinked collagen/chitosan scaffold(or collagen scaffold)was significantly more than that on the 5 mmol/L genipin crosslinked collagen/chitosan scaffold(P<0.05).(6)Results have suggested that genipin can adjust the physical and chemical properties of collagen/chitosan scaffolds within a certain extent.Among them,collagen/chitosan scaffold crosslinked with 3 mmol/L genipin has good mechanical properties,resistance to enzymatic hydrolysis and biocompatibility.
作者
张佳颖
索海瑞
徐铭恩
王玲
Zhang Jiaying;Suo Hairui;Xu Mingen;Wang Ling(School of Automation,Hangzhou Dianzi University,Hangzhou 310018,Zhejiang Province,China;Key Laboratory of Medical Information and 3D Bioprinting of Zhejiang Province,Hangzhou 310018,Zhejiang Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2022年第28期4477-4482,共6页
Chinese Journal of Tissue Engineering Research
基金
国家重点研发计划(2017YFC1103400),项目负责人:徐铭恩
国家自然科学基金(61675059),项目负责人:王玲。
关键词
交联
胶原
壳聚糖
低温3D打印
京尼平
组织工程
支架
细胞活性
crosslinking
collagen
chitosan
low-temperature 3D printing
genipin
tissue engineering
scaffold
cell viability
