摘要
目的:通过观察长时间艾烟吸入对大鼠嗅黏膜上皮细胞的影响,探讨艾烟的安全性。方法:健康成年SD大鼠,雌雄各半,随机分为正常组、低浓度艾烟组(低烟组)、中浓度艾烟组(中烟组)和高浓度艾烟组(高烟组),每组8只。正常组常规饲养,不同浓度艾烟组分别置于不同浓度的艾烟环境中进行艾烟熏灸,4 h/次,2次/d,共持续90 d。对各组大鼠进行持续90 d的一般状态观察;通过找寻食物小球实验比较各组大鼠找寻食物的时间;用TUNEL法观察各组大鼠嗅黏膜上皮细胞凋亡情况;用免疫组织化学法检测大鼠鼻黏膜基底细胞增殖情况。结果:与正常组、低烟组比较,中烟组、高烟组大鼠行为活动减弱,且对刺激反应强烈,精神状态较差,寻找食物小球时间明显延长(P<0.01)。与正常组比较,低、中、高烟组大鼠鼻黏膜嗅上皮凋亡细胞、基底增殖细胞增多(P<0.01,P<0.05);中、高烟组大鼠鼻黏膜嗅上皮凋亡细胞、基底增殖细胞较低烟组明显增多(P<0.01);高烟组大鼠鼻黏膜嗅上皮凋亡细胞、基底增殖细胞较中烟组明显增多(P<0.01)。结论:(1)长时间的中、高浓度艾烟吸入会对嗅觉功能产生损害,降低大鼠嗅觉灵敏度;低浓度艾烟对嗅觉功能没有显著影响。(2)长时间的艾烟吸入可诱导嗅黏膜上皮细胞的凋亡,并促进基底细胞增殖。
Objective To observe the effect of long-term inhalation of moxa-smoke on olfactory epithelial cells in rats, in order to explore the safety of moxa-smoke inhalation(MSI). Methods A total of 32 SD rats(half male and half female) were randomly divided into 4 groups: normal, low concentration(LCMSI), medium concentration(MCMSI) and high concentration(HCMSI), with 8 rats in each group. Rats of the LCMSI, MCMSI and HCMSI groups were put into closed boxes which were filled with ignited moxa stick-released smoke at concentrations of(0.11±0.05)mg/m^(3),(0.23±0.05) mg/m^(3) and(0.53±0.05)mg/m^(3), respectively. The treatment was given 4 h each time, twice a day for 90 days. Rats of the normal group were fed routinely. The rats’ general state and behavior(including fur appearance, activities in cage, response to external stimuli, spirit, stool, diet and water drinking) were recorded, and the olfactory function was assessed by using latency of finding the buried food pellet(BFP) test. The number of apoptotic olfactory epithelial cells was counted after terminal labeling(TUNEL), and the proliferation of basal cells of the nasal mucosa was detected by BrdU incorporation immunohistochemical technique. Results The latency of BFP was significantly longer in the MCMSI and HCMSI groups than in the normal and LCMSI groups(P<0.01), and had no significant differences between the LCMSI and normal groups, and between the MCMSI and HCMSI groups(P>0.05). The numbers of the apoptotic olfactory epithelial cells and proliferative basal cell in the nasal mucus tissue were markedly more in the LCMSI, MCMSI and HCMSI groups than in the normal group(P<0.01, P<0.05), and obviously more in the MCMSI and HCMSI groups than in the LMCMSI group(P<0.01), and apparently more in the HCMSI group than in the MCMSI group(P<0.01). The general state observation showed that in the first 45 days, only yellowish fur and water intake increase were seen in rats of the 3 moxa smoke inhalation groups, while no obvious changes in rats of the LCMSI group, and decrease in activities, being sensitive to external stimulation and fiddle-footed, and lower spirit in rats of the MCMSI and HCMSI groups in comparison with rats of the normal group after 90 day’s MSI. Conclusion Long-term inhalation of medium and high concentrations of moxa smoke may cause a reduction of the olfactory sensitivity and an increase of apoptosis of olfactory epithelial cells and proliferation of basal cells.
作者
蒋志明
吴立斌
李小贾
赵丽娜
刘磊
王洁
余情
胡玲
吴子建
JIANG Zhi-ming;WU Li-bin;LI Xiao-jia;ZHAO Li-na;LIU Lei;WANG Jie;YU Qing;HU Ling;Wu Zi-jian(Graduate School,Anhui University of Chinese Medicine,Hefei 230012,China;College of Acupuncture and Tuina,Anhui University of Chinese Medicine,Hefei 230012;Institute of Acupuncture and Meridian,Anhui Academy of Chinese Medicine,Hefei 230038)
出处
《针刺研究》
CAS
CSCD
北大核心
2021年第10期857-862,共6页
Acupuncture Research
基金
国家“973计划”项目(2015CB554504)
安徽中医药大学校级项目(2016ts024)。
关键词
灸法
艾烟
嗅觉功能
嗅细胞凋亡
细胞增殖
安全性
Moxibustion
Moxa smoke
Olfactory function
Olfactory cell apoptosis
Cell proliferation
Safety
