摘要
目的:探讨艾灸烟热效应对皮肤全切伤模型大鼠创面愈合、新生毛细血管和组织修复的影响。方法:SD大鼠随机分为模型组、艾烟组、艾热组,每组24只。参考Balaji团队的方法复制全层皮肤切除伤大鼠模型。艾烟组在造模后即刻用艾烟熏灸创面,艾热组除去艾烟后艾灸创面,两组均25 min/次,每日1次,连续10 d。于造模后第1、3、5、7天观察创面颜色、脓液、腐肉、肉芽、愈合情况。于造模后第3、7天采用HE染色、Masson染色观察创面局部组织和胶原纤维,于造模后第10天观察创面基底层新生毛细血管。结果:与模型组比较,术后第1、3、5、7天艾烟组创面面积显著减小(P<0.001),术后第3、5天艾热组创面面积显著减小(P<0.001);艾烟组各时点的创面面积均小于艾热组(P<0.01,P<0.001)。术后第3、7天,与模型组比较,艾热组和艾烟组创面再上皮化率明显升高(P<0.001),炎性细胞计数明显降低(P<0.001);且艾烟组创面再上皮化率高于艾热组(P<0.05,P<0.001),炎性细胞计数低于艾热组(P<0.001)。术后第10天,与模型组比较,艾热组和艾烟组的创面基底层新生毛细血管数量和密度明显升高(P<0.05,P<0.001,P<0.01);且艾烟组高于艾热组(P<0.01,P<0.05)。结论:艾灸烟、热效应对全层皮肤切除伤大鼠创面愈合均具有促进作用,其机制可能与抑制炎性反应,促进胶原纤维、肉芽组织和毛细血管的生成有关。
Objective To investigate the effect of moxibustion on wound healing,new capillaries and tissue repair in rats with full thickness skin excision.Methods SD rats were randomly divided into control,moxa-fumigating and moxa-heating groups,with 24 rats in each group.The full-thickness skin excision injury model was replicated according to Balaji’s and colleague’s methods.Rats in the moxa-fumigating group received fumigation of moxa-smog at the wound surface(25 min/time)immediately after modeling through a smoke-heat separation device,and those of the moxa-heating group received conventional thermal stimulation of the ignited moxa over the wound(25 min/time)without moxa smog,once a day for 10 consecutive days.The wound color,pus,carrion,granulation,and healing status of the rats were observed.H.E.staining and Masson staining were used to observe the local tissues and collagen fibers of the wound on the 3rd and 7th days.Newly born capillaries at the wound surface were observed on the 10th day.Results Following modeling,the wound area of the moxa-fumigating group on the 1st and 7th day,and those of both moxa-fumigating and moxa-heating groups on the 3rd and 5th day were significantly reduced compared with the model group(P<0.001),and the wound area of the moxa-fumigating group was obviously smaller than that of the moxa-hea-ting group(P<0.01,P<0.001).On the 3rd and 7th day after modeling,the re-epithelialization rates were significantly increased(P<0.001),and the amounts of inflammatory cells were significantly reduced in both moxa-fumigating and moxa-heating groups(P<0.001).At the same time,the re-epithelialization rate of the moxa-fumigating group was higher than that of the moxa-heating group(P<0.05,P<0.001),and the inflammatory cell count of the moxa-fumigating group was lower than that of the moxa-hea-ting group(P<0.001).On the 10th day after the modeling,the number of new capillaries and capillary density in the wound basal layer were significantly increased in both moxa-fumigating and moxa-heating groups(P<0.05,P<0.001,P<0.01),and were notably higher in the moxa-fumigating group than in the moxa-heating group(P<0.01,P<0.05).Conclusion Both fumigation and heating of moxibustion can promote wound healing in rats with full-thickness skin excision injury,which may be related to their effects in controlling the inflammatory response and promoting the production of collagen fibers,granulation tissue and capillaries.
作者
张帆
吴立斌
刘磊
王敏君
吴子建
胡玲
ZHANG Fan;WU Li-bin;LIU Lei;WANG Min-jun;WU Zi-jian;HU Ling(College of Graduate,Anhui University of Chinese Medicine,Hefei 230012,China;Acupuncture and Meridian Research Institute of Anhui Academy of Chinese Medicine,Hefei 230038)
出处
《针刺研究》
CAS
CSCD
北大核心
2021年第7期575-579,585,共6页
Acupuncture Research
基金
国家重点基础研究发展计划项目(No.2015CB554504)
安徽中医药大学校级探索科研项目(No.2016ts024)。
