摘要
目的本研究旨在探讨口腔鳞癌组织(Oral squamous cell carcinoma,OSCC)中含Sushi重复蛋白X连锁2(Sushi repeat containing protein X-linked 2,SRPX2)及Ras相关蛋白31(Ras-related protein 31,Rab31)的表达情况,并深入分析两蛋白与疾病发生发展的相关性及临床意义,为今后口腔鳞癌新型靶向药物的研发、预后指标的寻找提供新方向。方法利用Oncomine数据库比较分析SRPX2和Rab31在OSCC组织和正常口腔黏膜组织(Normal oral mucosa,NOM)组织的表达,收集哈尔滨医科大学附属第一医院2010年—2020年期间保存的OSCC组织标本68例及2018年—2020年间的非癌症患者NOM 30例,应用免疫组织化学染色检测SRPX2、Rab31在组织中的表达。根据检测结果进一步分析OSCC患者SRPX2和Rab31的相关性及两者阳性表达率与临床病理特征的关系。结果基于Oncomine数据库分析,OSCC组织中SRPX2和Rab31的阳性表达率均高于NOM组织中表达(P<0.05)。SRPX2在OSCC组织中表达高于NOM组织表达(70.6%vs.33.3%,P=0.001);Rab31在OSCC组织中表达同样高于NOM组织表达(66.2%vs.40.0%,P<0.05)。在OSCC组织中SRPX2与Rab31的表达具有相关性(P<0.05)。SRPX2和Rab31的表达与肿瘤分化程度、淋巴结转移相关(P<0.05)。Kaplan-Meier生存分析显示SRPX2、Rab31与OSCC患者不良预后相关(P<0.05)。结论OSCC组织中SRPX2、Rab31显著过表达并存在相关性,提示了两者可以联合作为OSCC预后评估的重要指标、新的药物靶点,为OSCC的治疗带来曙光。
Objective The objective of this study was to explore the expression of Sushi repeat containing protein X-linked 2(SRPX2)and Ras-related protein 31(Rab31)in oral squamous cell carcinoma(OSCC)and in-depth analysis of the relationship between the two proteins and the occurrence or development of the disease as well as clinical significance,to provide a new direction for the future development of new targeted drugs and the search for prognostic indicators for OSCC.Methods The expression of SRPX2 and Rab31 in OSCC and normal oral mucosal(NOM)tissues was analyzed by Oncomine database.A total of 68 cases of OSCC and 30 cases of NOM tissues of non-cancer patients were collected.Immunohistochemical staining was used to detect the expression of SRPX2 and Rab31 in the OSCC and NOM tissues.The correlation between the expression of SRPX2 and Rab31 as well as the relationship between their positive expression and the clinicopathological characteristics of OSCC patients were further analyzed.Results Based on the analysis of the Oncomine database,the expression of SRPX2 and RAB31 in OSCC tissues was higher than that in the NOM tissues.The expression of SRPX2 in OSCC tissues was higher than that in NOM tissues(0.6%vs.33.3%,P<0.05).The expression of Rab31 in OSCC tissues was also higher than that in NOM tissues(66.2%vs.40.0%,P<0.05).The expression of SRPX2 and Rab31 was significantly positively correlated in OSCC tissues(P<0.05).The expression of SRPX2 and Rab31 was also related to the degree of tumor differentiation and lymph node metastasis.Kaplan-Meier survival analysis showed that SRPX2 and RAB31 were related to the poor prognosis of patients with OSCC.Conclusion The significant overexpression and correlation of SRPX2 and RAB31 suggest that SRPX2 and RAB31 can be combined as an important indicator and a new drug target for the prognosis evaluation of OSCC,bringing hope to the treatment of OSCC.
作者
王哲
祝率
刘欢
付蔷
胡腾龙
WANG Zhe;ZHU Shuai;LIU Huan;FU Qiang;HU Tenglong(Department of Oral and Maxillofacial Surgery,The First Affiliated Hospital of Harbin Medical University,Harbin 150001,China)
出处
《实用肿瘤学杂志》
CAS
2021年第5期414-419,共6页
Practical Oncology Journal
基金
黑龙江省自然科学基金(编号:H2017029)。
