摘要
目的探讨Rab3A相互作用蛋白(Rab3IP)在食管癌组织中的表达,并分析其与临床资料及预后的关系。方法采用免疫组织化学染色和Western blotting检测Rab3IP在109例食管癌组织及其癌旁组织中的表达,分析Rab3IP表达方式与临床病理特征的关系;用Kaplan-Meier法分析Rab3IP表达与食管癌患者总体生存期和无瘤生存期的关系,Cox比例风险模型分析影响患者生存预后的因素。结果癌组织Rab3IP高表达率高于癌旁组织(P<0.05),癌组织Rab3IP相对表达量高于癌旁组织(P<0.05)。两组患者肿瘤直径、浸润深度、淋巴结转移及TNM分期比较,差异有统计学意义(P<0.05)。单因素和多因素分析显示,Rab3IP表达是影响食管癌患者术后总体生存时间的独立预后因素(P<0.05),低表达组术后总体生存时间高于高表达组(P<0.05)。Rab3IP表达是影响食管癌患者术后无瘤生存时间的独立预后因素(P<0.05),低表达组术后无瘤生存时间高于高表达组(P<0.05)。结论食管癌组织Rab3IP呈高表达,Rab3IP可作为食管癌预后评估的指标。
Objective To detect the expressions of Rab3A interacting protein(Rab3IP)in esophageal cancer tissues and to explore their correlations with the clinicopathological features and prognosis of the patients.Methods The expressions of Rab3IP in 109 esophageal cancer tissues and paired adjacent tissues was detected using immunohistochemical staining and western blot,respectively.The correlations of different patterns of Rab3IP expressions with the clinicopathological features of the patients were analyzed.Kaplan-Meier survival analysis was used to analyze the over-all survival and recurrence-free survival of the patients in relation with Rab3IP expressions.Cox regression were applied to analyse its prognostic value.Results Rab3IP were highly expressed in 70.6%(77/109)in the esophageal cancer tissues compared to their paired adjacent tissues 52.3%(57/109),the difference was statistically significant(P<0.05).The expression of TRab3IP were correlated with tumor size,invasion depth,lymph node metastasis,and TNM staging(P<0.05).Univariate and multivariate analysis showed that the expression of Rab3ip was an independent prognostic factor for overall survival time of esophageal cancer patients after operation(P<0.05),and the overall survival time of low expression group was higher than that of high expression group(P<0.05).The expression of rab3ip protein was an independent prognostic factor for tumor-free survival time in patients with esophageal cancer(P<0.05),and the tumor free survival time in the low expression group was higher than that in the high expression group(P<0.05).Conclusion Rab3IP are high expression in esophageal cancer tissues and can serve as new prognostic biomarkers for esophageal cancer.
作者
张岚
周利娟
肖旭阳
Lan Zhang;Li-juan Zhou;Xu-yang Xiao(Department of Thoracic Surgery,The first affiliated hospital of Jinzhou Medical University,Liaoning 121000 China;College of Nursing,Jinzhou Medical University,Liaoning 121000,China)
出处
《中国现代医学杂志》
CAS
2019年第24期32-37,共6页
China Journal of Modern Medicine
基金
辽宁省科学技术计划(No:201602327)
关键词
食管肿瘤
免疫组织化学
预后
esophageal neoplasms
immunohistochemistry
prognosis
