摘要
目的:建立HPLC法测定10种潮州凤凰茶叶中咖啡因的含量。方法:以Agilent ZORBAXEclipse XDB-C18为分析柱,样品进样体积为10μL。流动相A为0.2%的乙腈溶液,流动相B为55%甲醇,流速为1ml/min,柱温箱为30℃,紫外检测器检测波长272nm。结果:在所选条件下,样品中咖啡因与其它组分分离良好。在咖啡因浓度为0.02~0.16mg/mL范围内与峰面积有良好的线性关系,相关线性系数达到0.99999,加标回收率97.75%~99.83%之间,平均回收率为98.65%,RSD=0.8177%。10种凤凰单丛茶咖啡因含量在20.50-31.00mg/g之间。结论:该方法快速准确,可用于茶叶中咖啡因含量的测定。
AIM: To establish an HPLC method for the content determination of caffeine in ten kinds of Fenghuang tea from Chaozhou. METHODS: We taked Agilent ZORBAXEclipse XDB-C18 as analytical column, the inject volume was 10μL, and taked 0.2% acetonitrile solution as the mobile phase A and 55% methanol solution as the mobile phase B, flow rate was 1ml/min, the column temperature was 30℃,and the detection wavelength was 272nm.Under the selected conditions. RESULTS: The caffeine got an ideal separation with other compounds. The peak time of caf⁃ feine has a good liner relation with the peak area of caffeine when the concentration of caffeine range of 0.02 to 0.16mg/mL, and the relevant linear coefficient reached 0.99999(n= 5). In addition, the adding standard recoveries were 97.75%~99.83%, the average recoveries were 98.65%, and RSD=0.8177% .The caffeine content of ten kinds of Fenghuang Dancong tea ranged from 20.50 to 31.00mg/g.CONCLUSION: This method was quick and accurate. Therefor it was suitable for the determination of caffeine in tea.
作者
刘志聪
林丽丽
LIU Zhicong(School of Life sciences and Food Engineering,HanshanNormoal Universiy,ChouZhou 521000,China)
出处
《安徽农学通报》
2021年第17期159-162,共4页
Anhui Agricultural Science Bulletin
基金
广东省粤东药食资源功能物质与治未病研究重点实验室(2021B1212040015)。
