摘要
[目的]探讨ω-3多不饱和脂肪酸(ω-3PUFAs)对炎症性肠病(IBD)患者外周血单核细胞的影响。[方法]采集60例活动期IBD患者外周血标本,分离外周单个核细胞,随机分为三组,每组20份,分别以or 3PUFAs二十碳五烯酸(EPA)、二十二碳六烯酸(DHA),饱和脂肪酸硬脂酸(SA)处理48 h;另收集同期在新疆医科大学第五附属医院体检的健康志愿者血液标本20份,获取单个核细胞,细胞培养重悬,作为空白组。四组细胞均在48h后观察各细胞形态的变化;流式细胞仪检测CD4^(+)CD25^(+)FoxP3^(+)、CD4^(+)CD25^(+)CD127-T细胞数量;半定量反转录聚合酶链式反应法(RT-PCR)法检测细胞CD80、CD86 mRNA水平;酶联免疫吸附试验法(ELISA)测定细胞上清液白介素-12(IL-12)、L-10、肿瘤坏死因子-α(TNF-α)水平。[结果]空白组、SA组CD4^(+)CD25^(+)FoxP3^(+)、CD4^(+)CD25^(+)CD127-T细胞数量均显著高于EPA组和DHA组(P<0.05),SA组CD4^(+)CD25^(+)FoxP3^(+)、CD4^(+)CD25^(+)CD127-T细胞数量显著低于空白组(P<0.05);空白组.SA组细胞上清液CD80.CD86 mRNA显著高于EPA组和DHA组(P<0.05),SA组细胞上清液CD80.CD86 mRNA显著低于空白组(P<0.05);空白组、SA组细胞上清液IL-12、TNF-α水平显著高于EPA组与DHA组(P<0.05),IL-10水平显著低于EPA组与DHA组(P<0.05),SA组细胞上清液IL-12、TNF-α水平显著低于空白组,IL-10水平显著高于空白组(P<0.05);但EPA组与DHA组以上各指标比较差异均无统计学意义(P>0.05)。[结论]ω-3PUFAs可调节IBD免疫紊乱,维持免疫平衡,减轻炎症反应,促进受损细胞膜修复,或可能成为IBD治疗研究的新方向。
[Objective]To explore efcts of or ω-3 polyunsaturated fatty acids(ω-3PUFAs)on inflammatory bowel disease(IBD)and its mechanism.[Methods]Peripheral blood samples were collected from 60 patients with active IBD,and peripheral mononuclear cells were isolated.They were randomly divided into three groups.20 in each group.Respectively they were treated withorω-3PUFAs eicosapentaenoic acid(EPA),docosahexaenoic acid(DHA)and saturated fatty acid stearic acid(SA)for 48 hours;In addition,20 blood samples of healthy volunteers who underwent physical examination in our hospital in the same period were collected to obtain mononuclear cells.Cell culture was resuspended as the blank group.The morphological changes of cells in the four groups were observed after 48 hours;The number of CD4^(+)CD25^(+)FoxP3^(+)and CD4^(+)CD25^(+)CD127-T cells was detected by flow cytometry;The mRNA levels of CD80 and CD86 were detected by semi quantitative reverse transcription polymerase chain reaction(RT-PCR);Interleukin-12(IL-12),IL,-10 and tumor necrosis factor α(TNF-α)level in cell supernatant were measured by enzyme-linked immunosorbent assay(ELISA).[Results]The numbers of CD4^(+)CD25^(+)FoxP3^(+)and CD4^(+)CD25 CD127-T cells in the blank group and the SA group were higher than those in the EPA group and the DHA group(P<0.05).The numbers of CD4^(+)CD25^(+)FoxP3^(+)and CD4^(+)CD25^(+)CD127-T cells in the SA group was lower than that in the blank group(P<0.05).The CD80 and CD86 mRNAs in supernatants in the blank group and the SA group were higher than those in the EPA group and the DHA group(P<0.05).The CD80 and CD86 mRNAs in supernatants in the SA group were lower than those in the blank group(P<0.05).The concentration of IL-12 and TNF-α in cell supernatant in the blank group and SA group was higher than that in the EPA group and the DHA group(P<0.05),while IL-10 concentration was lower than that in the EPA group and the DHA group(P<0.05).The concentration of IL-12 and TNF-α in cell supernatant in the SA group was lower than that in the blank group,.while IL-10 concentration was higher than that in the blank group(P<C0.05).There was no significant difference in the above indexes between the EPA group and the DHA group(P>0.05).[Conclusion]ω-3 PUFAs can regulate IBD immune disorder,maintain immune balance,reduce inflammatory response,promote the repair of damaged cell membrane,or may become a new direction of IBD treatment research.
作者
冯晓玲
高鸿亮
FENG Xiao-ling;GAO Hong-liang(Department of Gastroenterology,the Fifth Affiliated Hospital of Xinjiang Medical University,830011)
出处
《医学临床研究》
CAS
2021年第7期978-982,共5页
Journal of Clinical Research
基金
新軀维吾尔自治区自然科学基金资助项目(编号:2019D01C276)。
