摘要
为探究甘油三酯(TG)含量对猪精液冷冻保存效果的影响,本实验测定公猪精浆TG含量,并采用常温(17℃)和超低温冷冻(-196℃)2种方式对精液进行保存,测定精液保存时间和精子质量指标。结果显示:精液TG含量与冷冻保存精液精子活率负相关(P<0.05),与冷冻保存精子活力、快速前进运动精子和慢速前进运动精子存在负相关趋势(0.05<P<0.1);精浆高TG(0.81 mmol/L)组的果糖、过氧化氢、总蛋白含量高于低TG(0.26 mmol/L)组(P<0.05),冷冻保存后高TG组精子活率低于低TG组(P<0.05),精子活率和活力下降比率高于低TG组(P<0.05)。可见,精浆中较高浓度的TG含量可能对猪精液冷冻保存存在不利影响。
In order to explore the effect of triglyceride(TG)content on the effect of pig semen cryopreservation,boar semen with different triglyceride content was stored at 17℃and cryopreservation(-196℃).TG level,storage time and sperm index were determined.The results showed that semen TG content was significantly negatively correlated with cryopreserved sperm viability,and there was a negative correlation trend(0.05<P<0.1)with cryopreserved sperm motility,fast-moving sperm and slow-moving sperm.The content of fructose,H2O2 and total protein in the seminal plasma with high TG content group(0.81±0.18 mmol/L)was significantly higher(P<0.05)than that in the low TG group(0.26±0.03 mmol/L).After cryopreservation,the sperm viability of the high TG content group was significantly(P<0.05)lower than that of the low TG group,and the sperm viability and motility decline rate were also significantly higher(P<0.05)than the low TG group.The higher TG content in seminal plasma may have an adverse effect on the cryopreservation of pig semen.
作者
甘麦邻
刘麟
郑婷
赵雪
郭宗义
沈林園
潘红梅
张亮
王金勇
朱砺
GAN Mailin;LIU Lin;ZHENG Ting;ZHAO Xue;GUO Zongyi;SHEN Linyuan;PAN Hongmei;ZHANG Liang;WANG Jinyong;ZHU Li(College of Animal Science and Technology,Sichuan Agricultural University,Sichuan Chengdu 611130,China;Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,Sichuan Agricultural University,Sichuan Chengdu 611130,China;Chongqing Academy of Animal Science,Chongqing 402460,China)
出处
《中国畜牧杂志》
CAS
北大核心
2021年第6期181-186,共6页
Chinese Journal of Animal Science
基金
国家重点研发计划项目(2018YFD0501004)
国家现代农业产业技术体系四川生猪创新团队项目(SCSZTD-3-008)
四川省科技支撑计划项目(2016NYZ0050)
重庆市社会事业与民生保障科技创新专项项目(cstc2017-zdyf0247)。
关键词
猪
精液
甘油三酯
常温保存
冷冻保存
Boar
Semen
Triglyceride
Storage at 17℃
Cryopreservation
