摘要
实验旨在研究β-1,3-葡聚糖的不同投喂方式对凡纳滨对虾(Litopenaeus vannamei)生长、血清代谢和抗亚硝酸氮应激能力的影响。选用480尾初体重(0.43±0.01) g的凡纳滨对虾,随机分为4组,即G0(全程投喂基础饲料)、G1组(全程投喂0.1%β-1,3-葡聚糖饲料)、G2组(0.1%β-1,3-葡聚糖饲料7d+基础饲料7d循环)和G3组(0.1%β-1,3-葡聚糖饲料14d+基础饲料14d循环)。在养殖84d后,应用亚硝酸钠进行120h亚硝酸氮应激实验。结果显示,各实验组凡纳滨对虾生长性能和全虾营养成分没有显著性差异。在养殖84d后, G2和G3组凡纳滨对虾肝胰腺脂肪酶活性显著高于G0和G1组(P<0.05), G1、G2和G3组凡纳滨对虾血清胆固醇和甘油三酯含量显著高于G0组(P<0.05), G3组凡纳滨对虾肌肉脂多糖/?-1,3-葡聚糖结合蛋白(LGBP)、酚氧化物酶原(proPO)和超氧化物歧化酶(SOD)mRNA表达显著高于G0和G1组(P<0.05)。亚硝酸氮应激120h, G1、G2和G3组凡纳滨对虾累计死亡率显著低于G0组(P<0.05), G3组凡纳滨对虾累计死亡率显著低于G0、G1和G2组(P<0.05)。在亚硝酸氮应激120h后,与G0组相比, G1、G2和G3组凡纳滨对虾血清总蛋白含量显著升高(P<0.05),葡萄糖含量、谷丙转氨酶和谷草转氨酶活性显著降低(P<0.05);G3组凡纳滨对虾肌肉LGBP、proPO和SOD mRNA表达显著高于G0 (P<0.05), G1组凡纳滨对虾肌肉丝氨酸蛋白酶(SP)mRNA表达显著高于G0、G2和G3组(P<0.05)。结果表明, 14d间隔投喂0.1%β-1,3-葡聚糖可能通过促进能量代谢和LGBP、proPO和SOD mRNA表达提高凡纳滨对虾抗亚硝酸氮应激能力。
To investigate the effects of dietaryβ-1,3-glucan on growth performance,serum metabolite,immune-related gene and resistance to nitrite stress in Litopenaeus vannamei,0.1%β-1,3-Glucan was supplemented to a basal diet(control,G0)with different feeding schedules:permanentlyβ-1,3-glucan diet(G1),7 daysβ-1,3 glucan diet-7 days of control diet(G2)and 14 daysβ-1,3 glucan diet-14 days of control diet(G3).After the feeding trial,these shrimps were exposed to nitrite-N(20 mg/L)for 120h.The results showed that dietaryβ-1,3-glucan had no significant effect on growth performance and whole-body composition after 84d feeding trial(P>0.05).The activity of lipase in hepatopancreas in G2 and G3 groups was significantly higher than that in G0 and G1 groups(P<0.05).The shrimp in G1,G2 and G3 groups had significantly higher hemolymph triacylglycerols and cholesterol levels than G0 group(P<0.05).Higher expression levels of LPS/b-glucan binding protein(LGBP),Prophenoloxidase(proPO)and superoxide dismutase(SOD)were observed in G3 group compared to G0 and G1 groups(P<0.05).After 120h nitrite-N stress,the mortality in G3 group was significantly lower than that of G0,G1 and G2 groups(P<0.05).Nitrite stress significantly reduced the levels of GLU,GOT and GPT and significantly enhanced hemolymph level.G3 group had significantly higher expression levels of LGBP,proPO and SOD compared to G0 group(P<0.05).The expression level of serine proteinase in G1 group was significantly higher than that in G0,G2 and G3 groups(P<0.05).L.vannamei fed diet G3 can improve nitrite resistance probably by accelerating energy metabolism and increasing expression levels of LGBP,proPO and SOD.
作者
赵红霞
陈冰
莫文艳
黄文
黄燕华
曹俊明
ZHAO Hong-Xia;CHEN Bing;MO Wen-Yan;HUANG Wen;HUANG Yan-Hua;CAO Jun-Ming(Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture,Guangzhou 510640,China;Guangdong Key Laboratory of Animal Breeding and Nutrition,Guangzhou 510640,China;Guangzhou Fishtech Biotechnology Co.,Ltd.,Guangzhou 510640,China)
出处
《水生生物学报》
CAS
CSCD
北大核心
2021年第3期593-600,共8页
Acta Hydrobiologica Sinica
基金
广东省现代农业产业技术体系建设项目(2018LM083和2019KJ115)
科技创新战略专项资金(高水平农科院建设,201614TD)资助。
