摘要
褐腐病菌Monilinia fructicola是引起多种果树褐腐病的重要病原菌,前期研究发现该病原菌对甲基硫菌灵的抗性与Tub2蛋白的多个氨基酸变异有关。为明确不同类型菌株的温度适应性及乙霉威是否对所有抗性类型菌株均具有抑菌活性,本研究测定了敏感型菌株S及3种抗性类型包括R(E198A)、R(E198Q)及R(F200Y)型菌株的温度敏感性及其对甲基硫菌灵、多菌灵和乙霉威的敏感性差异,同时分析了不同突变类型菌株对苯并咪唑类药剂和乙霉威的交互抗性。结果表明,4种类型菌株的最适生长温度为21~22℃,其中R(E198Q)型菌株的最适生长温度最低,且其在低温13℃和高温28℃条件下,生长速率显著低于其他3类菌株,而S、R(E198A)及R(F200Y)型菌株在所有温度条件下的生长速率均无显著差异。3种抗性类型菌株对甲基硫菌灵的敏感性有所差异,其中R(E198Q)型表现较为敏感,R(F200Y)型在低浓度下与R(E198A)型无明显差异,但在高浓度下对甲基硫菌灵表现较为敏感;对多菌灵的敏感性差异与甲基硫菌灵相似;只是R(E198Q)和R(F200Y)型菌株对多菌灵比甲基硫菌灵更为敏感。乙霉威的敏感性测定结果表明,R(E198A)型菌株表现敏感,S类型表现为不敏感性,R(E198Q)及R(F200Y)两种类型均表现为抗性,且抗性水平差别不大。线性相关分析结果表明,仅R(E198A)型菌株和S型菌株对苯并咪唑类杀菌剂和乙霉威敏感性存在显著负相关性(甲基硫菌灵和乙霉威:r=−0.992,p=0.000;多菌灵和乙霉威:r=−0.982,p=0.001)。综合分析结果表明,在3类抗性菌株中,仅R(E198A)型菌株对苯并咪唑类杀菌剂和乙霉威存在负交互抗性。
Monilinia fructicola is an economically important pathogen,causing brown rot on many fruit trees.Previous studies revealed that the resistance in this pathogen to thiophanate-methyl was associated with multiple amino acid variations in the Tub2 protein.To understand the temperature adapatability and the activity of diethofencarb in differrent types of isolates,the sensitivities to thiophanate-methyl,carbendazim and diethofencarb were investigated in the sensitive type isolates S and three types of resistant isolates including R(E198A),R(E198Q)and R(F200Y),among which the cross-resistance of different types to MBCs and diethofencarb were analyzed.The results showed that the optimum growth temperature of the four types was ranged from 21 to 22℃,among which the R(E198Q)isolates had the lowest optimum growth temperature,and its growth rate was significantly smaller than the other three types under the conditions of 13℃and 28℃.While no significant difference was observed in the growth rate of S,R(E198A)and R(F200Y)isolates under all temperature conditions.The three resistant types showed different sensitivities to thiophanate-methyl,among which the R(E198Q)type was least resistant,and the R(F200Y)type was no difference from the R(E198A)type at low concentration but significantly less resistant under high concentration of thiophanate.The difference in sensitivity of three resistant types to carbendazim was similar to that of thiophanate-methyl,only in which the R(E198Q)and R(F200Y)types were more sensitive to carbendazim than thiophanate-methyl.The sensitivity test to diethofencarb showed that the R(E198A)isolates were sensitive,and S isolates were insensitive,while the other two types of R(E198Q)and R(F200Y)showed resistance with little difference.The results of linear correlation analysis showed a significant negative correlation between the sensitivity to benzimidazole fungicides and diethofencarb only in R(E198A)type and S type,(thiophanate-methyl and diethofencarb:r=−0.992,p=0.000;carbendazim and diethofencarb:r=−0.982,p=0.001).All the results mentioned above showed that only the R(E198A)type had negative cross-resistance to methyl-benzimidazole carbamates(MBCs)and diethofencarb among the three types of resistant isolates.
作者
陈凤平
郑钰珑
周挺
张玥
黄中乔
刘西莉
CHEN Fengping;ZHENG Yulong;ZHOU Ting;ZHANG Yue;HUANG Zhongqiao;LIU Xili(State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Institute of Tobacco Science,Fujian Provincial Tobacco Company,Fuzhou 350003,China;Department of Plant Pathology,China Agricultural University,Beijing 100193,China;Department of Plant Pathology,Northwest A&F University,Yangling 712100,Shaanxi Province,China)
出处
《农药学学报》
CAS
CSCD
北大核心
2021年第1期97-106,共10页
Chinese Journal of Pesticide Science
基金
“十三五”国家重点研发计划项目(2016YFD0201305)
福建农林大学科技创新专项项目(CXZX2019011G).
关键词
褐腐病菌
苯并咪唑类杀菌剂
甲基硫菌灵
多菌灵
乙霉威
负交互抗性
Monilinia fructicola
methyl benzimidazole carbamates
thiophanate-methyl
carbendazim
diethofencarb
negative cross-resistance
