摘要
研究设计合成一种新型噻菁染料Dye1,在PBS缓冲溶液中实现对人血清白蛋白(HSA)高选择性检测。向Dye1-PBS体系中加入HSA后,通过检测荧光,发现Dye1在444 nm处的荧光强度显著增加,说明此体系可应用于HSA检测。优化检测条件后,在HSA浓度为3~30 mg/L时,HSA与Dye1有良好的线性关系(R^(2)=0.9886),检出限为1.8 mg/L,且反应迅速达到平衡,可以实现快速、高选择性检测HSA。
A thiacyanine dye(Dye1)was synthesized to realize high selectivity detection of human serum albumin(HSA)in PBS buffer.The fluorescence intensity of Dye1 at 444 nm could be increased significantly with increasing concentrations of HSA in PBS.The system could be used to detect of HSA.The detection conditions were optimized.The linear relationship of Dye1 with HSA was from 0 to 30 mg/L(R^(2)=0.9886),the detection limit was 1.8 mg/L,and response time was short that could achieve rapid and high selectivity detection of HSA.
作者
赵晗
张颖琳
师晓栋
丁春光
于丽佳
ZHAO Han;ZHANG Ying-lin;SHI Xiao-dong;DING Chun-guang;YU Li-jia(National Center for Occupational Safety and Health,NHC,Beijing 102308,China)
出处
《化学试剂》
CAS
北大核心
2021年第2期136-140,共5页
Chemical Reagents
基金
国家自然科学基金资助项目(21907021)。
关键词
噻菁染料
人血清白蛋白
荧光光谱
高选择性
检测
合成
thiacyanine dye
human serum albumin(HSA)
fluorescence spectroscopy
high selectivity
detection
synthesis
