摘要
为构建具有自主知识产权的大麦突变体库,从而为大麦种质创新和解析作物主要农艺性状形成机理提供材料,本研究通过^(60)Co-γ和甲基磺酸乙酯(EMS)复合诱变处理,构建了自育大麦品种鄂大麦934的突变体库,包含982株与对照存在明显差异且性状无分离、可遗传的变异株,涉及叶片、茎秆、穗子、籽粒、分蘖、株型、抽穗期、结实率等多个农艺性状。通过扫描电镜对叶宽突变体的叶片细胞进行显微观察,发现突变体在细胞水平上变化各异。本研究构建的突变体库为开展大麦功能基因组学研究奠定了基础。
To construct our own mutant library and provide materials for germplasm innovation and illustration of mechanisms involving in agronomic traits in barley and crops,mutant population of barley Edamai 934,which was an elegent cultivar bred by Hubei Academy of Agricultural Sciences,was constructed using^(60)Co-γcombined with EMS.Totally 982 mutants were identified,which showed significant and hereditable changes as compared with the wild type Edamai 934 on one of the following traits,leaf,stem,spike,seed,tiller,plant architecture,heading date,seed setting rate,and so on.Further cytological analysis of leaf width mutants showed that size and morphology of cells from flag leaf of different mutants varied a lot,suggesting that different mechanisms may involve in.This study lays foundation for barley functional genomics.
作者
秦丹丹
许甫超
徐晴
彭严春
葛双桃
董静
焦春海
QIN Dandan;XU Fuchao;XU Qing;PENG Yanchun;GE Shuangtao;DONG Jing;JIAO Chunhai(Institute of Food Crops,Hubei Academy of Agricultural Science,Wuhan,Hubei 430064;Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement,Wuhan,Hubei 430064)
出处
《核农学报》
CAS
CSCD
北大核心
2021年第2期262-270,共9页
Journal of Nuclear Agricultural Sciences
基金
国家重点研发计划(2018YFD1000706、2018YFD1000700)
国家自然科学基金(31771774)
国家大麦青稞产业技术体系(CARS-05)
国家留学基金委访问学者(201808420082)。
关键词
大麦
突变体库
理化诱变
叶宽
barley
mutant population
physical and chemical mutagenesis
leaf width
