摘要
目的探讨运动训练对轻度认知功能障碍(MCI)模型小鼠学习记忆能力减退及海马区病理学改变的影响。方法选取健康雄性16月龄昆明小鼠90只,随机分为对照组、MCI模型组和运动干预组,每组30只。小鼠被给予颈背部皮下注射D-半乳糖(500 mg/kg)、亚硝酸钠(45 mg/kg)及三氯化铝(40 mg/kg)灌胃,连续3周,制作MCI小鼠模型。模型制作成功后,对运动干预组小鼠给予跑台运动干预3周。检测3组小鼠行为学变化、海马匀浆胆碱乙酰转移酶(ChAT)、乙酰胆碱酯酶(AchE)浓度、β-淀粉样蛋白42(Aβ42)含量,观察3组海马组织病理学改变。结果与对照组比较,MCI模型组逃避潜伏期、第1次穿越原平台位置时间延长,在目标象限活动时间缩短(P<0.05);与MCI模型组比较,运动干预组逃避潜伏期、第1次穿越原平台位置时间缩短,在目标象限活动时间延长(P<0.05)。与对照组比较,MCI模型组海马ChAT表达水平明显降低,AchE、Aβ42表达水平明显升高(P<0.05);与MCI模型组比较,运动干预组海马ChAT表达水平明显升高,AchE、Aβ42表达水平明显降低(P<0.05)。MCI模型组海马CA1区锥体细胞密度下降,部分细胞核固缩、深染,可见少量细胞凋亡,神经纤维排列稍稀疏;银染示锥体细胞稍稀疏,细胞内神经元纤维稍增粗、模糊,细胞外未见Aβ斑。运动干预组海马CA1区锥体细胞密度增加,细胞结构及神经纤维清晰、有序,未见凋亡细胞;银染示CA1区锥体细胞排列有序,细胞内神经元纤维清晰。结论运动训练可以明显改善MCI模型小鼠海马组织的病理学改变,提高其学习记忆能力。
Objective To investigate the effects of exercise training on learning and memory impairment and hippocampal pathological changes in mild cognitive impairment(MCI)mice model.Methods A total of 90 healthy male Kunming mice aged 16 months were randomly divided into three groups:control group(n=30),MCI model group(n=30)and exercise intervention group(n=30).Mice were subcutaneously injected with D-galactose(500 mg/kg)and sodium nitrite(45 mg/kg)and intragastric gavage of aluminum trichloride(40 mg/kg)for 3 weeks to replicate the MCI mouse model.After the model was successfully established,the mice in the exercise intervention group were given running-platform exercise intervention for 3 weeks.Behavioral changes,concentration of hippocampal homogenate ChAT and AchE,the content of Aβ42 and hippocampal pathological changes in each group were detected.Results Compared with the control group,escape latency(EL)and time of first crossing the original platform position(TFCOPP)were prolonged,and the time of activity in the target quadrant(TATQ)was shortened in the MCI model group(P<0.05).Compared with the MCI model group,EL and TFCOPP were shortened and TATQ was prolonged in the exercise intervention group(P<0.05).Compared with the control group,the expression level of ChAT in hippocampus of MCI model group was significantly decreased,while the expression level of AchE and Aβ42 was significantly increased(P<0.05).Compared with the MCI model group,the expression level of ChAT in the hippocampus of the exercise intervention group was significantly increased,while the expression level of AchE and Aβ42 was significantly decreased(P<0.05).In the MCI model group,the density of pyramidal cells in the hippocampal CA1 region was decreased,part of the nuclei were shrunk and deeply stained,a small number of cells were observed to undergo apoptosis,and the arrangement of nerve fibers was slightly sparse.As for silver staining,in the MCI model group,the pyramidal cells in the hippocampus CA1 area were slightly sparse,the intracellular neurofibrils were slightly thickened and blurred,and no amyloid plaques was observed outside the cell.In the exercise intervention group,the density of pyramidal cells in the hippocampus CA1 was increased,and the cell structure and nerve fibers were clear and orderly.No apoptotic cells were observed.The pyramidal cells in the hippocampal CA1 area were arranged in an orderly manner,and the intracellular neurofibrils were clear.Conclusion Exercise training can significantly improve the pathological changes in the hippocampus of MCI model mice,and improve their learning and memory ability.
作者
方侃
吕光辉
汪新体
艾志兵
王启斌
FANG Kan;LYU Guang-hui;WANG Xin-ti;AI Zhi-bing;WANG Qi-bin(Department of Pharmacy,Taihe Hospital Affiliated to Hubei University of Medicine,Shiyan,Hubei 442000,China;Department of Neurology,Taihe Hospital Affiliated to Hubei University of Medicine,Shiyan,Hubei 442000,China)
出处
《解放军医药杂志》
CAS
2021年第2期5-9,共5页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
基金
2018年十堰市科学技术研究与开发立项项目(18Y36)。
