摘要
本试验旨在研究丁酸梭菌对哺乳母猪免疫和抗氧化的影响,选取健康经产长白母猪32头(胎次和预产期相近),随机分为4组,每组8个重复,每个重复1头。对照组饲喂基础日粮,试验组分别在基础日粮基础上添加250、500、1000 mg/kg丁酸梭菌。预试期7 d,试验期为28 d(产前7 d至21 d断奶)。结果表明:哺乳第1天,1000 mg/kg丁酸梭菌组血清中IgA、IgM和IgG含量均显著高于对照组;哺乳第21天,250、500、1000 mg/kg丁酸梭菌组IgA含量均显著高于对照组,500 mg/kg丁酸梭菌组IgM和IgG含量均为最高;哺乳第1、10、21天,1000 mg/kg丁酸梭菌组IL-6和IL-1β含量较对照组均显著降低;哺乳第1、10、21天,各丁酸梭菌组超氧化物歧化酶活性均显著高于对照组,丙二醛含量均显著低于对照组;哺乳第1、21天,500、1000 mg/kg丁酸梭菌组的过氧化氢酶含量较对照组均显著升高;哺乳第21天,500 mg/kg丁酸梭菌组总抗氧化能力和谷胱甘肽过氧化物酶活性显著升高;哺乳第1天和第21天,1000 mg/kg丁酸梭菌组粪便中大肠杆菌的数量显著低于对照组。由此得出,在妊娠后期和哺乳期饲粮中添加丁酸梭菌可以显著提高母猪免疫功能和抗氧化能力,降低粪便中大肠杆菌的数量,且添加量为500~1000 mg/kg时效果明显。
The purpose of this experiment was to study the effect of Clostridium butyricum on immune and antioxidant of suckling sows.Thirty-two healthy long-term white sows were selected(the parity and the expected date of delivery were similar),and randomly divided into 4 groups,8 repeats in each group and 1 pig in each repeat.The control group was fed basic diet,and the experimental group was supplemented with 250,500 and 1000 mg/kg Clostridium butyrate on the basis of basic diet.The pre-test period is 7 days and the test period is 28 days(7 d before birth to 21 d weaning).The result shows that on the first day of breastfeeding,the serum IgA,IgM and IgG levels in the 1000 mg/kg C.butyricum group were significantly higher than those in control group;On the 21st day of breastfeeding,the IgA content of the 250,500 and 1000 mg/kg C.butyricum group was significantly higher than that of the control group,and the IgM and IgG content of the 500 mg/kg C.butyricum group were the highest;On the 1st,10th and 21st day of breastfeeding,the IL-6 and IL-1βlevels in the 1000 mg/kg C.butyricum group were significantly lower than those in control group;On the 1st,10th and 21st day of breastfeeding,the activity of superoxide dismutase in the C.butyricum addition group was significantly higher than that in control group,and the content of malondialdehyde was significantly lower than that in control group;On the 1st and 21st day of breastfeeding,the content of the catalase in the 500 and 1000 mg/kg C.butyricum group was significantly different from that of control group;On the 21st day of breastfeeding,the 500 mg/kg C.butyricum group had the highest total antioxidant capacity and glutathione peroxidase activity;On the 1st and 21st day of breastfeeding,the number of E.coli in the feces of the 1000 mg/kg C.butyricum group was significantly lower than that of control group.It can be concluded that the addition of Clostridium butyricum to the diet of late pregnancy and lactation can significantly improve the sow's immune function and antioxidant capacity,and reduce the number of E.coli in feces,and the effect is obvious when the dosage is 500~1000 mg/kg.
作者
冯云龙
王晶莹
于淼
刘萱
马倩
刁新平
FENG Yunlong;WANG Jingying;YU Miao;LIU Xuan;MA Qian;DIAO Xinping(College of Animal Science and Technology,Northeast Agricultural University,Heilongjiang Harbin 150030,China;Animal Husbandry Station,Heilongjiang Acheng 150300,China)
出处
《中国畜牧杂志》
CAS
北大核心
2021年第2期170-176,共7页
Chinese Journal of Animal Science
基金
国家自然科学基金(31672429)。
关键词
丁酸梭菌
免疫
抗氧化
大肠杆菌
哺乳母猪
Clostridium butyricum
Immunity
Antioxidation
E.coli
Nursing sows
