摘要
目的:观察蛭龙活血通瘀胶囊及其拆方对动脉粥样硬化(AS)大鼠内皮素(ET)-1和一氧化氮(NO)的影响,并探讨其组方的合理性。方法:随机将110只健康雄性SD大鼠分为11组,分别为空白组、模型组、阿托伐他汀组、全方组和拆方1~7组,每组各10只,除空白组外其余各组均制备AS大鼠模型。阿托伐他汀组大鼠予以阿托伐他汀钙片干预,全方组予以黄芪、桂枝、大血藤、地龙、水蛭干预,拆方1组予以黄芪、桂枝、大血藤干预,拆方2组予以黄芪、地龙、水蛭干预,拆方3组予以黄芪干预,拆方4组予以桂枝、地龙干预,拆方5组予以桂枝、水蛭干预,拆方6组予以大血藤、地龙干预,拆方7组予以大血藤、水蛭干预,于实验第120天后检测各组大鼠治疗前后的血清ET-1、NO水平。结果:蛭龙活血通瘀胶囊全方组较拆方组降低血清ET-1浓度和提高血清NO浓度的作用更为明显,诸药中影响大鼠血清ET-1、NO含量最显著的是黄芪。结论:蛭龙活血通瘀胶囊全方组中多种药味合用后,对降低AS大鼠血清ET-1和提高NO含量有明显的协同作用,方中多种药物均可保护内皮功能,且影响大鼠血清ET-1、NO含量最显著的药味是黄芪。
Objective: To investigate the effect of Zhilong Huoxue Tongyu capsules and its components on endothelin-1( ET-1) and nitric oxide( NO) in rats with atherosclerosis( AS) and the rationality of such prescription. Methods: A total of 110 healthy male Sprague-Dawley rats were divided into blank group,model group,atorvastatin group,whole prescription group,and component 1-7 groups,with 10 rats in each group,and all rats except those in the blank group were used to establish a rat model of AS. The rats in the atorvastatin group were treated with atorvastatin calcium tablets;the rats in the whole prescription group were treated with Astragalus membranaceus,Ramulus Cinnamomi,Sargentodoxa cuneata,Pheretima,and leech;the rats in the component 1 group were treated with Astragalus membranaceus,Ramulus Cinnamomi,and Sargentodoxa cuneata;the rats in the component 2 group were treated with Astragalus membranaceus,Pheretima,and leech;the rats in the component 3 group were treated with Astragalus membranaceus;the rats in the component 4 group were treated withRamulus Cinnamomi and Pheretima;the rats in the component 5 group were treated with Ramulus Cinnamomi and leech;the rats in the component 6 group were treated with Sargentodoxa cuneata and Pheretima;the rats in the component 7 group were treated with Sargentodoxa cuneata and leech. The serum levels of ET-1 and NO were measured before treatment and on day 120 of the experiment. Results: Compared with the components of Zhilong Huoxue Tongyu capsules,its whole prescription had a significantly better effect in reducing serum ET-1 and improving serum NO,and among the above drugs,Astragalus membranaceus had the most significant effect on the serum levels of ET-1 and NO. Conclusion:The combination of multiple medicines in the whole prescription of Zhilong Huoxue Tongyu capsules has a marked synergistic effect on reducing serum ET-1 and improving NO in AS rats. Various medicines in this prescription can protect endothelial function,among which Astragalus membranaceus has the most significant effect on the serum levels of ET-1 and NO.
作者
郑利珠
张诗意
廖慧玲
ZHENG Lizhu;ZHANG Shiyi;LIAO Huiling(School of Integrated Traditional Chinese and Western Medicine,Southwest Medical University,Luzhou 646000,Sichuan,China)
出处
《湖南中医杂志》
2020年第12期139-141,146,共4页
Hunan Journal of Traditional Chinese Medicine
基金
四川省科技应用基础研究计划项目(2019YJ0649)。
