摘要
目的探讨应用改良的Hulth法建立猕猴膝骨关节炎动物模型的可行性及可靠性。方法实验组:选用猕猴的10只膝关节,应用改良Hulth手术造模,即切断膝关节内侧副韧带,切除前十字韧带、内侧半月板,并在股骨内侧髁关节面切除部分软骨。对照组:选用实验组猕猴的对侧10只膝关节,只切开皮肤、关节囊,不破坏关节腔结构。对构建的猕猴膝骨关节炎模型分别在造模后2、4、6个月从膝关节功能、X线片、MRI、关节镜下结构、切开肉眼所示大体情况、膝关节股骨软骨组织切片等方面评估骨关节炎造模情况。结果对照组在术后2、4、6个月的X线片、MRI及切开肉眼观察关节腔与术前无明显差异。实验组在造模后2个月X线片示内侧关节间隙狭窄,膝关节力线内翻;造模后4个月,X线片示关节软骨下骨硬化、关节周缘骨赘形成,髌骨骨赘增生;造模后6个月,X线片示关节软骨下骨硬化、关节周缘骨赘增生进一步加重。实验组造模后2个月,膝关节MRI表现为内侧关节间室狭窄,股骨内侧髁可见软骨造模缺损区,关节腔少许积液;造模后4个月,MRI表现为关节腔积液增多,髁软骨下骨髓水肿;造模后6个月,MRI出现外侧半月板Ⅱ级退变信号、部分区域软骨变薄、软骨下骨髓水肿、骨囊性变及关节积液中的一种或多种征象。造模后6个月,关节镜检查及肉眼均见到软骨毛糙、无光泽、有裂纹,局部凸凹不平,髁间窝滑膜增生。造模术后6个月,组织切片示软骨表层裂隙,出现局部缺损,软骨退变。造模后6个月,实验组膝关节活动度无明显改变,猕猴在笼内行走活动减少,有轻度跛行。实验组的10只造模膝关节在6个月后均造模成功。结论通过改良的Hulth手术可以在造模后6个月有效建立猕猴膝骨关节炎动物模型。
Objective To investigate the feasibility and reliability of establishing an macaque model of knee osteoarthritis by modified Hulth surgical methods.Methods Ten knee joints of macaque were selected as experimental group.The modified Hulth surgical method was performed for the experimental group by cutting the anterior cruciate ligament and medial collateral ligament,removing medial meniscus,and part of cartilage on the articular surface of the medial femur condyle.Another 10 knee joints on the opposite side of experimental knees were selected as the control group.Sham operation was performed by cutting the skin and capsule without destroying the structures of joint.At 2,4 and 6 months after modeling,the changes of knee function and behavior,knee X-ray,MRI,visual observation,arthroscopy observation and Histological section of osteochondral bone were collected to evaluate the results of osteoarthritis modeling.Results In the control group,there was no significant difference in X-ray,MRI and visual observation of the joint cavity at 2,4 and 6 months after operation.In the experimental group,X-ray showed medial joint space narrowing and knee force line varus at two months after modeling;formation of subchondral osteosclerosis,periarticular osteophytes,and patella osteophytes was observed at 4 months after modeling;further aggravation of subchondral osteosclerosis and periarticular osteophytosis was found at six months after modeling.At 2 months after modeling,the MRI showed stenosis of medial articular compartment,and defect area of cartilage modeling could be seen in the medial condyle of femur,and there was a little effusion in articular cavity.At 4 months after modeling,the MRI of knee joint showed articular cavity effusion increased and subcondylar bone marrow edema.At six months after modeling,MRI showed one or more signs of grade II degeneration of the lateral meniscus,cartilage thinning in some areas,subchondral bone marrow edema,bone cystic degeneration,and joint effusion.At six months after the modeling,arthroscopy and macroscopic observation showed that the cartilage was coarse,lackluster,cracked,locally bumpy and synovial hyperplasia of the intercondylar fossa was found.At six months after modeling,tissue sections showed cracks,local defectsin and degeneration on the surface of cartilage.At six months after the modeling,the knee range of motion did not change significantly in the experimental group,and the walking activity of the rhesus monkey in the cage was reduced,accompanied by mild claudication.The 10 modeled knee joints of the experimental group were successfully modeled in 6 months.Conclusion The modified Hulth operation can effectively establish an animal model of knee osteoarthritis in macaques six months after modeling.
作者
李滔
陈仲
凌斌
徐俊
修光辉
毕鑫
朱守艳
赵庆刚
蒋俊良
李俊宏
Li Tao;Chen Zhong;Ling Bin;Xu Jun;Xiu Guanghui;Bi Xin;Zhu Shouyan;Zhao Qinggang;Jiang Junliang;Li Junhong(Department of Bone and Trauma Surgery,Yunnan Second People's Hospital,Kunming 650021,China;Shanghai Tongji University Medical School,Shanghai 200331,China;Department of Radiology,Yunnan Second People's Hospital,Kunming 650021,China)
出处
《中华骨科杂志》
CAS
CSCD
北大核心
2020年第24期1679-1687,共9页
Chinese Journal of Orthopaedics
基金
云南省科技厅徐俊专家工作站(2017IC025)
云南省科技厅昆明医科大学联合专项重点项目(2019FE001-170)。
关键词
猕猴
骨关节炎
膝
模型
动物
Macaca mulatta
Osteoarthritis,knee
Models,animal
