摘要
目的观察新型冠状病毒肺炎康复者恢复期血浆中IgG抗体在亚甲蓝/光化学病毒灭活法和冰冻保存前后滴度的变化。方法随机留取50份2020年2~3月新冠肺炎康复者恢复期血浆的亚甲蓝/光化学灭活前后血浆样辫,冰冻保存,间隔4个月后对其进行SARS-CoV-2 IgG抗体滴度检测。结果亚甲蓝/光化学灭活前后的恢复期血浆IgG抗体滴度差异无统计学意义(P>0.05),冰冻保存4个月后血浆中SARS-CoV-2 IgG抗体滴度变化亦无统计学意义(P>0.05)。结论目前新冠肺炎康复者恢复期血浆的制备程序对血浆中IgG抗体滴度无显著性影响,提示制备流程合理。
Objective To observe the changes of IgG antibody titers in plasma of convalescent COVID-19 patients before and after methylene blue/photochemical virus inactivation and freezing preservation.Methods Fifty plasma samples donated by convalescent patients from February to March 2020 were randomly collected and frozen for detection of SARS-CoV-2 IgG antibody titer after 4 months interval.Results There was no significant difference in plasma IgG antibody titer in recovery period before and after methylene blue/photochemical inactivation(P>0.05),and there was no significant difference in plasma SARS-CoV-2 IgG antibody titer after frozen storage for up to 4 months(P>0.05).Conclusion At present,the preparation process of convalescent plasma of convalescent patients has no significant effect on IgG antibody titer in plasma,suggesting that the preparation process is reasonable.
作者
郑雪
包安裕
瞿珍
许婷婷
谢松丽
Zheng Xue;Bao Anyu;Qu Zhen(Wuhan Blood Center,Wuhan 430030,China;Clinical Laboratory,Renmin Hospital of Wuhan University,Wuhan 430060,China)
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2020年第6期721-723,共3页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
湖北省卫生健康委科研资助项目(No.WJ2019H349)
武汉市卫生健康委科研资助项目(No.WX19Q02)。
