摘要
目的研究地肤子提取物石油醚相、乙酸乙酯相、正丁醇相对于α-葡萄糖苷酶所具有的抑制效应及抑酶动力学性质。方法采用PNPG底物模型筛选地肤子提取物以对α-葡萄糖苷酶的活性部位加以抑制,同时通过Lineweave-Burk双倒数方式了解其动力学性质。结果地肤子正丁醇相和乙酸乙酯相具有较高的α-葡萄糖苷酶抑制活性。地肤子正丁醇相的抑制效应显著超过阳性对照组阿卡波糖,正丁醇相和乙酸乙酯相的半数抑制浓度(IC 50)依次是743.54μg/mL、1339.65μg/mL。动力学试验的相关结果揭示,地肤子正丁醇相和乙酸乙酯相的抑酶活性效应类型是非竞争型的。结论地肤子正丁醇相对于α-葡萄糖苷酶具有较强的抑制效应,可以据此加以研究利用。
Objective To study the inhibition ofα-glucosidase and its reaction kinetics by the three polar moieties of petroleum ether fraction,ethyl acetate fraction,n-butanol fraction of Kochia scoparia seeds.Methodsα-glucosidase inhibitory fraction in Kochia scoparia extracts was screened by PNPG substrate model,and the dynamic properties were analyzed by Lineweaver-Burk double reciprocal method.Results The Kochia scoparia extracts were all had certain inhibitory effect onα-glycosidase,in which inhibitory effect of n-butanol fraction was obviously higher than that of positive control acarbose,IC 50 were 743.54μg/mL,1339.65μg/mL,respectively.The results of kinetic test showed that the inhibition of n-butanol fraction and ethyl acetate fraction were non competitive.Conclusion The n-butanol extract had significant inhibitory effect onα-glucosidase,which had good value of development and utilization.
作者
张卫
冯松浩
张雨
王晶晶
李健
王洪涛
ZHANG Wei;FENG Songhao;ZHANG Yu;WANG Jingjing;LI Jian;WANG Hongtao(Department of Pharmacy,Zhengzhou Railway Vocational and Technical College,Henan Zhengzhou 451460,China)
出处
《临床研究》
2020年第12期16-18,共3页
Clinical Research
基金
河南省教育厅高等学校重点科研项目指导计划(18B350010)。
