摘要
基于二维相关中红外光谱法,建立一种快速判别牛奶中四环素的分析方法。分别配置20个纯牛奶和20个掺杂四环素牛奶样品(浓度区间为0.005~30 mg/L)。室温下,在400~4000 cm^-1范围内采集牛奶样品的常规一维谱数据。以牛奶中掺杂四环素浓度为外扰,分别在1500~1700 cm^-1和3250~3450 cm^-1波段区间内构建同步和异步二维相关谱,研究其相关谱特性。通过对同步谱中交叉峰的正负以及异步谱中相同位置是否存在交叉峰,推断1550 cm^-1和1640 cm^-1处所对应的基团吸收峰来自于牛奶中的四环素,是由C==N键和C==O键的伸缩振动引起的。同时,在3310 cm^-1处的波峰也来自于四环素,是由N--H拉伸及弯曲振动引起的。结果表明,对二维相关中红外光谱特性的研究,可以定性判别牛奶中是否掺杂四环素。
Based on the two-dimensional correlation mid infrared spectroscopy,a rapid method for the identification of tetracycline in milk was established.20 pure milk samples and 20 tetracycline adulterated milk samples(0.005~30 mg/L)were prepared respectively.At room temperature,the conventional one-dimensional spectral data of milk samples were collected in the range of 400~4000 cm^-1.Taking the concentration of tetracycline in milk as the external perturbation,synchronous and asynchronous two-dimensional correlation spectra were constructed in the range of 1500~1700 cm^-1 and 3250~3450 cm^-1,respectively.The absorption peaks at 1550 cm^-1 and 1640 cm^-1 were supposed to come from the tetracycline in milk,which were caused by the stretching vibration of C==N and C==O bonds.At 3310 cm^-1,the peak also came from tetracycline,which was caused by the stretching and bending vibration of N--H bond.The results showed that the analysis of two-dimensional correlation mid infrared spectral characteristics can qualitatively determine whether the milk is adulterated with tetracycline.
作者
王宏璐
黄文静
黄志伟
原尚奇
解诗雨
吴楠
WANG Hong-lu;HUANG Wen-jing;HUANG Zhi-wei;YUAN Shang-qi;XIE Shi-yu;WU Nan(College of Engineering and Technology,Tianjin Agricultural University,Tianjin 300384,China)
出处
《天津农学院学报》
CAS
2020年第3期79-82,共4页
Journal of Tianjin Agricultural University
基金
大学生创新创业训练计划项目(201910061090)
国家自然科学基金项目(21607114,41771357)。
关键词
牛奶
四环素
中红外光谱
二维相关
milk
tetracycline
mid infrared spectroscopy
two-dimensional correlation
