摘要
木二糖是低聚木糖的重要组成部分。通过对S-Ca树脂、ZA-Ca树脂、分子筛、P-Ca树脂、Na+树脂、L-Na树脂对木二糖的静态吸附与解吸的比较,在筛选出一种具有较高吸附率与解吸量树脂的基础上,比较不同洗脱流速下不同体积分数乙醇梯度洗脱对木二糖洗脱量、纯度、得率的影响及最优流速下不同乙醇体积分数等梯度洗脱对木二糖洗脱量、纯度、得率的影响。结果表明,Na树脂是几种树脂中分离纯化木二糖的最佳树脂,最佳参数:流速为0.8 BV,经过2 BV的10%乙醇进行梯度洗脱的木二糖、总得率和总纯度分别为4.78 mg,62.8%和5.7%。
Xylobiose was main product of xylan by xylooligosccharide hydrolysis.The effects of static adsorption and desorption of macroporous resin-S-Ca,ZA-Ca,sieve chromatography,P-Ca,Na+and L-Na for xylobiose were compared.On the basis of screening the best macroporous resin with higher adsorbing rate and with desorbing amount,the effects of the desorbing quantity,purity and yield rate of xylobiose different eluting velocities by the desorption gradient of different eluting density of ethanol were studied,and the effects of desorbing quantity,purity and yield rate of xylobise by the same gradient of different eluting density under the best eluting velocity were also studied.The results showed that Na+was the best among the resin.To separate and purify xylobiose by Na+at 0.8 BV/h with 2 BV desorbing volume of ethanol concentration 10%ethanol gradient desorptionis proved to be the best,with the total quantity of elute xybiose,yield rate,the total purity reaching 4.78 mg,62.8%and 5.7%,insequence.
作者
隋明
周文
谢慧蓉
李俊儒
张凤英
SUI Ming;ZHOU Wen;XIE Huirong;LI Junru;ZHANG Fengying(Sichuan Technology&Business College,Dujiangyan 611830;College of Light Industry,Textile&Food Engineering,Sichuan University,Chengdu 610065)
出处
《食品工业》
CAS
北大核心
2020年第9期64-68,共5页
The Food Industry
关键词
木二糖
静态吸附
动态吸附
柱分离
xybiose
static adsorption
dynamic adsorption
column separation and purifiation
