摘要
目的研究电磁脉冲(EMP)辐照后海马神经元形态、大鼠海马神经源素2(Ngn2)和甲基CpG结合蛋白2(MeCP2)蛋白表达水平的改变。方法成年健康雄性SD大鼠随机分为对照组、EMP 7 d组、EMP 14 d组(n=12)。采用HE染色检测大鼠海马神经元的形态、Western blot法检测大鼠海马神经元Ngn2、MeCP2、脑源性神经营养因子(BDNF)、突触后致密蛋白95(PSD95)的蛋白水平,免疫荧光组织化学染色检测大鼠海马神经元MeCP2、Ngn2的表达。结果与对照组比较,EMP 7 d组和EMP 14 d组锥体细胞减少,神经元边界模糊,细胞核轮廓模糊不清。与EMP 7 d组相比,EMP 14 d组海马神经元结构破坏更严重;与对照组比较,EMP 14 d组和EMP 7 d组Ngn2、BDNF、PSD95含量降低,而MeCP2表达增加。结论EMP暴露导致大鼠海马组织神经元病理损伤,MeCP2表达增加,Ngn2、PSD95、BDNF表达降低。
Objective To investigate the alterations in hippocampus neuron morphology,the expression of neurogenin 2(Ngn2)and methyl-CpG-binding protein 2(MeCP2)in hippocampus after electromagnetic pulse(EMP)irradiation.MethodsAdult healthy male SD rats were randomly divided into control group,EMP 7-day group,and EMP 14-day group(n=12).Hippocampus neuron morphology was tested by HE staining;the expression of Ngn2 and MeCP2 were measured by Western blotting and fluorescent immunohistochemistry;the protein levels of brain-derived neurotrophic factor(BDNF)and postsynaptic density protein 95(PSD95)were measured by Western blotting.Results Compared with the control group,the number of pyramidal cells in the EMP 7-day group and EMP 14-day group was reduced,the neuron boundary was blurred,and the nuclear outline was blurred.Compared with the EMP 7-day group,the hippocampus neuron structure in the EMP 14-day group was more severely damaged.Compared with the control group,the expression of Ngn2,BDNF,and PSD95 significantly decreased,while the expression of MeCP2 increased in the EMP 14-day group and EMP 7-day group.Conclusion EMP exposure leads to the pathological damage of hippocampus neurons in rats,increases MeCP2 expression and decreases Ngn2,PSD95,BDNF expression.
作者
王文莹
李江静
张霞婧
王爽
张贺铭
高昌俊
孙绪德
WANG Wenying;LI Jiangjing;ZHANG Xiajing;WANG Shuang;ZHANG Heming;GAO Changjun;SUN Xude(Weifang Medical University,Weifang 261053;Department of Anesthesiology,Tangdu Hospital,Air Force Medical University,Xi'an 710038;Department of Anesthesiology,Xi’an No.4 Hospital,Xi’an 710004,China)
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2020年第5期425-429,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
陕西省自然科学基础重大研究项目(2016ZDJC-16)。
