摘要
采用酶联免疫吸附法测定血清睾酮、精子顶体酶、睾丸组织中超氧化物歧化酶和丙二醛含量;CASA系统检测精子的活力指标;TUNEL法检测睾丸组织中生精细胞的凋亡情况。结果表明,大鼠生殖系统所受到的即时损伤与修复与辐照脉冲的场强和脉冲数有关,场强越高,脉冲数越大,损伤越明显,修复越困难。结果提示,在实验条件下,场强的影响比脉冲次数的影响更显著。
The testosterone, superoxide dismutase （SOD）, malondialdehyde （MDA）, and acrosin activity were measured by ELISA. The cell apoptosis of testicular was observed by TUNEL. The sperm motility was analyzed by CASA. The results showed that the harms caused by W-EMP exposure, and the subsequent recovery were dependent on the intensity and pulse number of W-EMP. With the increase of electric intensity and pulse number the impact of W-EMP exposure increased, which leaded to a slower recovery rate. The results indicates that compared with the pulse number, the electric intensity in present work has much stronger impact on the reproductive system.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
2017年第1期13-20,共8页
Journal of Radiation Research and Radiation Processing
基金
国家重点基础研究基金项目(2011CB503701)
国家自然科学基金青年项目(31600675)资助~~
关键词
宽频谱电磁脉冲
生殖系统
精子活力
血清睾酮
顶体酶
Wide-band electromagnetic pulse, Reproductive system, Sperm motility, Serum testosterone, Acrosin
