摘要
目的通过3D打印技术构建负载二甲双胍的聚乙烯醇(PVA)/β-磷酸三钙(β-TCP)骨组织工程支架,初步探讨其应用潜能。方法向PVA溶液中掺人二甲双胍,利用低温3D打印技术制备载药的PVA/β-TCP/二甲双胍(PTM)支架,评估支架的微观形态、孔径及孔隙率、吸水率、二甲双胍的释放规律、细胞毒性、细胞黏附和增殖。结果PTM支架具有相互连通的孔隙结构,与不载药(PT)支架比较,支架表面粗糙,吸水率明显提高(P<0.05),但药物的掺入并不影响支架的孔径(P>0.05)和孔原率(P>0.05)。PTM支架内的二甲双胍在前5天出现较快的释放,随后进人缓慢释放阶段,至14天时释放率约为74%。通过支架浸提液培养细胞24h,细胞生存率大于90%。细胞在支架上黏附良好,随着时间推移,细胞增殖活性逐步提升,第5天时,PTM支架的细胞增殖活性明显大于PT支架(P<0.05)。结论3D打印PTM支架符合骨组织工程支架的表征,具备二甲双胍级释能力,支架生物相容性好,可提高细胞的增殖活性,有望在骨再生领域发挥潜力。
Objective To coat metformin on the PV A(poly vinyl alcohol)/β-tricalcium phosphate saffld prepared by 3D printing and to explore its application potentiality in bone tisue enginering.Methods Metformin was incorporated into the PVA solution,and a drug-loaded PVA/β-TCP/MET(PTM)scaffold was prepared by low-temperature 3D printing technology,and assessing the microstructure,pore size and porosity,water absorption,release of metformin,cell viability,attachment and proliferation.Results PTM scaffold had interconneeted pore structure,cormpared with non-medicated(PT)scaffold,had rougher surfaces and significantly improved wa-ter absoption(P<0.05),but the incorporation of drugs did not afet the pore size(P>0.05)and porosity(P>0.05).The metformin in the PTM scaffold appeared to be released rapidly in the first 5 days,and then entered the slow release stage.The release rate was about 74%by 14 days.The cells were cultured with the scaffold extract for 24 hours,and the cell survival rate of each group was greater than 90%.The cells adhered well to the scaffold,and the cell proliferation activity gradually increased with time.On the 5 days,the cell prolif-eration activity of the PTM saffold was significantly greater than that of the PT seaffold(P<0.05).Conclusion The 3D printed PTM scaffold is in line with the characterization of bone tissue engineering scaffolds,and has metformin sustained-release capability.PTM scaffold has good biocompatibility,which can increase the proliferation activity of cell.The PTM scaffold is expected to show its potential in the field of bone regeneration.
作者
戴桓琰
孙一丹
郑剑莹
费凡
韩冰
Dai Huanyan;Sun Yidan;Zheng Jianying(Hospital of Stomatology,Jilin University,Jilin Provincial Key Laboratory of Tooth Development and Jaw Bone Remodeling and Regeneration,Jilin 130021,China)
出处
《医学研究杂志》
2020年第8期47-51,共5页
Journal of Medical Research
基金
国家自然科学基金资助项目(81771041)
吉林省科技厅科技发展计划项目(20190701035GH)。
关键词
3D打印
支架
二甲双胍
Β-磷酸三钙
骨组织工程
3D printing
Scaffold
Metformin
β-trie alcium phosphate
Bone tissue engineering
