摘要
目的探究恶性胶质瘤U87细胞中微小RNA-147(miR-147)启动子区甲基化程度与miR-147表达量及细胞增殖的关系。方法使用0μmol/L(空白组)、4μmol/L以及16μmol/L的5′aza-dC培养基培养U87细胞,通过亚硫酸氢盐修饰后测序法(BSP法)检测每组细胞中miR-147启动子区甲基化情况;使用QPCR法检测各组miR-147表达量,并对细胞进行计数,计算U87细胞受抑制程度。结果空白组甲基化率为14.6%,miR-147相对表达量为0.29±0.02;4μmol/L组甲基化率为8.9%,miR-147相对表达量为0.66±0.03,有19.4%的细胞受抑制;16μmol/L组甲基化率为3.4%,miR-147相对表达量为1.09±0.05,有51.6%的细胞受抑制。各组miR-147相对表达量比较,差异具有统计学意义(P<0.001)。结论U87细胞中miR-147启动子区的甲基化程度改变可能是影响U87细胞中miR-147表达的因素之一,并且甲基化程度越低,U87细胞增殖受抑制越明显。
Objective To explore the relationship between the methylation with the promoter region of microRNA-147(miR-147)in glioblastoma U87 cells and the expression of miR-147 and cell proliferation.Methods U87 cells were cultured with 0 mol/L(blank group),4 mol/L,and 16 mol/L in 5′aza-dC medium.The methylation of the miR-147 promoter region in each group of cells was detected by Bisulfite Modified Sequencing(BSP)method.The expressions of miR-147 in each group were detected by QPCR method,and the cells were counted to calculate the inhibition rate of U87 cells.Results The methylation rate was 14.6%in blank group,and the relative expression of miR-147 was 0.29±0.02;the methylation rate was 8.9%in 4μmol/L group,the relative expression of miR-147 was 0.66±0.03,19.4%of the cells were inhibited;the methylation rate was 3.4%in 16μmol/L group,the relative expression of miR-147 was 1.09±0.05,and 51.6%of the cells were inhibited.The relative expressions of microRNA-147 in each group were statistically significant(P<0.001).Conclusion The degree of methylation degree of the miR-147 promoter region in U87 cells might be one of the factors affecting the expression of miR-147 in U87 cells,and the lower degree of methylation,the more significantly inhibition of U87 cell proliferation.
作者
武伟男
许小斌
连世忠
WU Weinan;XU Xiaobin;LIAN Shizhong(First Clinical Medical College of Shanxi Medical University,Taiyuan 030001,Shanxi,China;First Hospital of Shanxi Medical University,Taiyuan 030001,Shanxi,China)
出处
《中西医结合心脑血管病杂志》
2020年第15期2426-2429,共4页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
基金
山西省基础研究计划项目(自然)(No.2015011095)。
