摘要
目的通过检测MicroRNA92a在宫颈癌以及正常人血液中的表达量,分析其与临床病理参数之间的关系,初步探讨MicroRNA92a在宫颈癌中的作用机制。方法收集宫颈癌以及正常人血液各30例,采用实时荧光定量PCR方法检测宫颈癌及正常人血液中MicroRNA92a的表达情况,分析其与宫颈癌临床病理参数之间的关系。结果 MicroRNA92a在宫颈癌病人血液中的表达量明显高于正常人,差异有统计学意义(P<0.05),其表达水平与患者的年龄、肿瘤大小、大体类型、组织分化程度和是否转移无关(P>0.05)。结论 MicroRNA92a表达的失调可能与宫颈癌的发生发展有关,检测MicroRNA92a表达量对宫颈癌的早期诊断、预后和治疗方面有一定的指导意义。
Objective To study the expression of microRNA92 ain blood of patients with cervical cancer and in normal blood,to explore the correlations between microRNA92 aexpression and clinicopathological features and to study the mechanism of microRNA92 ain cervical cancer.Methods 30 blood specimens were collected from patiens with cervical cancer and healthy people..Quantitative real-time PCR（QRT-PCR）was used to measure the expression of microRNA92 ain the blood of patients with cervical cancer and in normal blood,and the correlations between microRNA92 aexpression and clinicopathological features were analyzed.Results MicroRNA92 aexpression in the blood of patients with cervical cancer was significantly higher than that of the normal blood（P〈0.05）.No correlation was observed between microRNA92 aexpresssion and age,the size of tumor,typesof tumor,tissue differentiation or metastasis（P〉0.05）.Conclusion The disorders of the MicroRNA92 aexpression may be related to the occurrence and development of cervical cancer,and the detection of MicroRNA92 aexpression in the blood of cervical cancer patients help to guide the early diagnosis,the prognosis and the treatment.
出处
《山东医学高等专科学校学报》
2015年第6期465-468,F0002,共5页
Journal of Shandong Medical College
