摘要
为研究不同分离株弓形虫棒状体蛋白ROP 16基因的遗传变异,扩增9种不同来源的弓形虫样品的ROP 16基因,并对其序列进行比对及构建进化树的分析。结果表明,弓形虫不同分离株ROP 16序列的同源性均在99.0%以上,但存在限制性位点多态性;用弓形虫ROP 16作为PCR-RFLP的标记分子,未能鉴别出传统的弓形虫Ⅰ型、Ⅱ型、Ⅲ型,却鉴定出一株特殊的猪源弓形虫分离株。弓形虫ROP 16基因的株间保守性提示其是一个用于预防弓形虫病的潜在疫苗候选分子,值得对其进行深入研究。
To study the heritable variation of ROP 16 gene(TgROP16)of different Toxoplasma gondii strain,this paper amplified and analyzed TgROP 16 gene sequences of the strains from different hosts and geographical locations.Phylogenetic analysis of TgROP 16 genes from the examined T.gondii isolates was conducted with MEGA 5.05 using the Maximum Likelihood method program.Variation in TgROP 16 sequences among the 9 examined T.gondii isolates were low with a homology of more than 99.0%.Phylogenetic analysis of the sequences of TgROP16 gene showed that the clustering of the 9 T.gondii isolates was not matched to the three classical genotypes.The results suggested that TgROP 16 gene might be a promising vaccine candidate against all genotypes of toxoplasmosis because of its highly conservation among different strains.
作者
黄勉
冯伟利
植广林
杨子鹏
左珂菁
杨晓颖
陈绚姣
袁浩
袁子国
HUANG Mian;FENG Weili;ZHI Guanglin;YANG Zipeng;ZUO Kejing;YANG Xiaoying;CHEN Xuanjiao;YUAN Hao;YUAN Ziguo(Guangzhou Zoo,Guangzhou 510070,China;College of Veterinary Medicine,South China Agricultural University,Guangzhou 51064,China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2020年第7期117-123,共7页
Journal of Agricultural Science and Technology
基金
国家重点研发计划项目(2018YFD0501200)
广州动物园专项项目(h2016141)
广东省重点领域研发计划项目(2019B020218004)
国家自然科学基金项目(31972707)
广东省自然科学基金项目(2019A1515011534)。
