摘要
弓形虫感染可引起严重的弓形虫病,而不同宿主感染的弓形虫株基因型差异较大,不同基因型弓形虫株的致病力及药物敏感性亦存在较大差异。目前,对弓形虫基因型的分析多选用限制性片段长度多态性PCR(PCR RFLP)、高度重复序列PCR、多重PCR和巢氏PCR等技术,扩增位点多选择B1、SAG2、HSP70、GRA6等遗传标记。传统的弓形虫基因型主要有3个,随着研究的不断深入,越来越多的基因型被发现。本文就弓形虫基因分型研究进展进行综述。
Toxoplasma gondii is a nucleated cell obligate parasite, and can cause severe toxoplasmosis. The genetypes of Toxoplasma gondii isolates of different host infections have significant differences, and the pathogenicity and sensitivity to drugs of different genotypes of Toxoplasma gondii isolates are also significantly different. At present, the analysis of Toxoplasma gondii gen otypes is performed by using PCRRFLP, PCR of highly repetitive sequences, multiple PCR and nested PCR techniques, and the multiple loci of amplification are usually B 1, SAG2, HSP70, GRA6 and other genetic markers. There are main 3 traditional geno types of Toxoplasma gondii, and along with the deepening and extension of research, more and more genotypes are found. This pa per reviews the advances in the research of Toxoplasma gondii genotypes.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2013年第2期202-204,共3页
Chinese Journal of Schistosomiasis Control
基金
国家自然科学基金(30960330)
关键词
弓形虫
基因
基因分型
Toxoplasma gondii
Gene
Genotyping
