摘要
从辣椒果实胎座中分离出60株内生细菌,平皿对峙培养发现13株细菌对辣椒白绢病菌(Sclerotium rolfsii Sacc.)有拮抗作用,其中6株对白绢病抑制率较高的生防菌对辣椒疫病(Phytophthora capsici)和辣椒炭疽病(Colletotrichum capsici)病原菌也有抑菌活性。16Sr RNA鉴定结果表明,菌株G242和T443为贝莱斯芽孢杆菌(Bacillus velezensis),菌株YZ12、G241、T151、T442为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。以单一菌株或菌株复配进行盆栽试验,结果 G241和T443复配后对辣椒白绢病的防效达到88.41%,高于单一菌株G241(29.14%)和T443(38.16%)的防效。
Sixty strains of endophytic bacteria were isolated from the fruit of placenta of capsicum,and in antagonistic assay 13 of them showed antagonistic effect on Sclerotium rolfsii Sacc.,the 6 most effective biocontrol strains also inhibited the growth of Phytophthora capsica and Colletotrichum capsici.Strain G242 and T443 were identified as Bacillus velezensis and strain YZ12,G241,T151,and T442 were verified as Bacillus amyloliquefaciens by 16S rRNA test.The planting experiments in the greenhouse were conducted on single strain or combining strains and the results indicated that the control effect of combining strains of G241 and T443 on Sclerotium rolfsii Sacc.reached to 88.41%which is much higher than G241(29.14%)and T443(38.16%)alone.
作者
吕翠
张亮
黄军
刘清术
郑井元
王运生
戴良英
陈武
LYU Cui;ZHANG Liang;HUANG Jun;LIU Qingshu;ZHENG Jinyuan;WANG Yunsheng;DAI Liangying;CHEN Wu(College of Plant Protection,Hunan Agricultural University,Changsha,Hunan 410128,China;College of Agromy,Hunan Agricultural University,Changsha,Hunan 410128,China;Department of Applied Fungi,Hunan Institute of Microbiology,Changsha,Hunan 410009,China;Institute of Vegetable,Hunan Academy of Agricultral Science,Changsha,Hunan 410125,China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第3期331-335,共5页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省自然科学基金项目(2019JJ40119、2019JJ40169、2019JJ40153)
湖南省科学技术厅重点研发计划项目(2019NK2192)。
关键词
辣椒
果实胎座
生防菌
辣椒白绢病
防效
capsicum
the placenta
biocontrol bacteria
pepper southern blight
biocontrol effect
