摘要
目的检测Epstein-Barr病毒(EBV)感染的传染性单核细胞增多症(IM)患儿外周血淋巴细胞亚群、细胞因子及Toll样受体7(TLR7)、TLR9的表达变化及其临床意义。方法选取2019年1月—2019年12月杭州市儿童医院收治的98例感染EBV致IM急性期患儿作为研究对象,另外选择50例EBV感染阴性儿童作为对照组。采用流式细胞术和酶联免疫吸附试验分别检测IM患儿急性期、恢复期(病程满1个月后复查)外周血淋巴细胞亚群和细胞因子水平。另外分离外周全血单个核细胞(PBMC),采用qRT-PCR检测TLR7和TLR9 mRNA。结果与对照组比较,IM急性期患儿外周血CD3+、CD8+、白细胞介素-1β(IL-1β)、IL-6、IL-8、IL-10、肿瘤坏死因子α(TNF-α)、γ干扰素(IFN-γ)、TLR7和TLR9 mRNA相对表达量升高(P<0.05);CD4^+、CD19^+、CD16^+56^+降低(P<0.05)。IM急性期患儿EBV DNA载量与血清IL-1β、TLR9 mRNA呈正相关(r s=0.247和0.348,P<0.05),与其他指标无关(P>0.05)。IM患儿在恢复期时,外周血CD3^+、CD8^+、IL-1β、IL-6、IL-8、IL-10、TNF-α、IFN-γ、TLR7和TLR9 mRNA相对表达量降低(P<0.05);CD4^+、CD19^+、CD16^+56^+升高(P<0.05)。结论监测IM患儿外周血淋巴细胞亚群、细胞因子及TLR7、TLR9的表达变化,尤其是重点关注血清IL-1β和TLR9 mRNA的表达,有助于了解病情进展和转归。
Objective To observe the difference of peripheral blood lymphocytes subsets,cycokines and toll like receptors 7(TLR7),TLR9 in infectious mononucleosis children infected with epstein-barr virus(EBV).Methods Ninety-eight IM children in acute phase and 50 healthy volunteers without EBV infection as control group in our hospital from January to December 2019 were enrolled into the study.The flow cytometry and ELISA methods were used to detected the percent of lymphocytes subsets and levels of IL-1β,IL-6,IL-8,IL-10,TNF-α,IFN-αand IFN-γ.Peripheral blood mononuclear cell(PBMC)were isolated from the blood,TLR7 mRNA and TLR9 mRNA in PBMC were detected by RT-qPCR.Results Compared with control group,CD3^+,CD8^+,IL-1β,IL-6,IL-8,IL-10,TNF-α,IFN-γ,TLR7 mRNA and TLR9 mRNA levels in acute IM children increased,while CD4+T,CD19+,CD16+56+declined(P<0.05).Spearman analysis showed that the load of EBV-DNA in acute IM children would be positively related with IL-1βlevel and TLR9 mRNA(r=0.247 and 0.348,P<0.05).At the recovery stage,CD3^+,CD8^+,IL-1β,IL-6,IL-8,IL-10,TNF-α,IFN-γ,TLR7 mRNA and TLR9 mRNA levels declined(P<0.05)and CD4^+,CD19^+,CD16^+56^+increased(P<0.05),compared with the acute IM stage.Conclusions There are some differences of peripheral blood lymphocytes subsets,cycokines and TLR7,TLR9 between acute IM stage and recovery IM stage,while especially the detection of IL-1βand TLR9 levels might be helpful to evaluate the development and clinical outcomes.
作者
祁正红
陈雪夏
林先耀
赵仕勇
Zheng-hong Qi;Xue-xia Chen;Xian-yao Lin;Shi-yong Zhao(Department of Infectious Diseases,Hangzhou Children’s Hospital,Hangzhou,Zhejiang 310014,China)
出处
《中国现代医学杂志》
CAS
2020年第12期51-56,共6页
China Journal of Modern Medicine
